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Plant Tissue Genomic DNA Isolation Kit

Details for Product No. ABIN2967295, Supplier: Log in to see
Application
DNA Isolation (DNAIso)
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Purpose Isolates highly pure, intact plant DNA
Sample Type Plant Tissue, Tissue Samples
Specificity Plants serve a vital role in human nutrition, biofuels, and the ecosystem. Isolating DNA from plants is exceptionally challenging compared to other organisms due to the presence of difficult to disrupt cell wall and compounds such as tannins, phenolics, and complex polysaccharides that can affect DNA quality and inhibit downstream reactions.
Characteristics Plant Genomic DNA Isolation kit enables the researchers to isolate genomic DNA from plant leaves, stalks, seeds, and roots. This kit utilizes an efficient combination of lysis buffer and enzymes that work in unison to disrupt the cell wall, eliminate plant specific contaminating compounds, and release free genomic DNA. DNA release from the cell is coupled with adsorption of DNA onto a silica spin-column under chaotropic conditions, eliminating the use of toxic organic compounds or solvents. This kit yields pure DNA that is suitable for various downstream molecular biology applications such as PCR, cloning, sequencing, DNA hybridization and Southern Blotting, etc.
Useful for PCR, Cloning, DNA hybridization, Southern Blotting
Components Buffer L [Lysis Buffer]
1 M DTT
Enzyme Mix
RNAse A
Buffer B [Binding Buffer]
Buffer W [Wash Buffer]
Buffer E [Elution Buffer]
Spin Columns/Collection Tubes
Comment

Further details regarding sample type: Fresh or dry leaf, root, and seed plant tissues

Restrictions For Research Use only
Storage RT/-20 °C
Expiry Date 24 months