You are viewing an incomplete version of our website. Please click to reload the website as full version.

DNA clean and concentrate High Throughput

Details for Product No. ABIN3071672, Supplier: Log in to see
Application
DNA Extraction (DEx), High-throughput Screening (HTS)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Purpose Chromatrap® 96 DNA Micro Elution Purification Kit for high throughput plates for ultra-pure DNA.
Brand Chromatrap®
Characteristics Chromatrap® 96-well high throughput plates for ultra-pure DNA purification. Using proprietary filtration media that offer much higher loadings of active material, assay times are under 5 minutes. Buffers are optimised to remove any unwanted impurities while providing efficient DNA recovery from samples. The Chromatrap® 96 DNA Micro Elution Purification Kit is designed for the purification and concentration of samples from PCR mixtures, ChIP samples and restriction enzyme Digestions.
Components
  • 2x Chromatrap® 96 DNA Micro Elution Purification plate (RT)
  • 2x 96-well collection plate (RT)
  • 2x 96-well elution plate (RT)
  • 100mL DNA Binding Buffer (RT)
  • 30mL DNA Wash Buffer (RT)
  • 2 mL DNA Elution Buffer (RT)
Material not included Additional materials required
  • Ethanol (96-100 % )
  • 3M sodium acetate pH 5.2
  • Centrifuge with plate rotor
  • Multi channel pipette
Comment

  • DNA samples ranging from 50 bp up to 23 kb can be purified with up to 98% recovery
  • Up to 10 μg of DNA can be recovered efficiently and quickly using a Chromatrap® 96 DNA Micro Elution Purification Kit

Assay Procedure

Protocol

Preparation step

Add ethanol (96-100 % ) to DNA Wash Buffer before use (120 mL ethanol to the DNA Wash buffer concentrate for a total volume of 150 ml). Note on the label that ethanol has been added.

Note all centrifugation steps are carried out at 3,000xg at RT.

  1. Add 5 volumes DNA Binding Buffer to 1 volume of sample and mix.

    DNA Binding Buffer contains an integrated pH indicator. DNA adsorption requires a pH of ≤7.5, and the pH indicator in the buffers will appear yellow in this range. If the pH is >7.5 the binding mixture will turn orange or violet meaning the pH of the sample exceeds the buffering capacity of the DNA Binding Buffer and DNA adsorption will be inefficient. In these cases, add 10 μL 3M sodium acetate, pH 5.2, to adjust the pH of the binding mixture. The colour of the mixture should turn yellow.
  2. Place a Chromatrap® 96 DNA Micro Elution Purification plate and position on to the 96-well collection plate provided.
  3. Transfer each sample to a corresponding well on the Chromatrap® 96 DNA Micro Elution Purification plate.
  4. Centrifuge at 3,000xg for 60 seconds at RT. Discard the flow through.
  5. Add 700 μL DNA Wash Buffer to each well and centrifuge at 3,000xg for 60 seconds at RT. Discard the flow through.
  6. Spin dry at 3,000xg for 30 seconds at RT to remove any remaining liquid from the plate.
  7. Transfer the Chromatrap® DNA Micro Elution Purification plate on to a clean supplied 96-well DNA Elution plate.
  8. To elute DNA, add 5-10 μL DNA Elution buffer to the centre of the frit in each well and incubate for 1 minute. Centrifuge at 3,000xg for 60 seconds to collect the eluted DNA.

Troubleshooting Guide and FAQs are given in the delivered protocol.

Restrictions For Research Use only
Storage RT
Storage Comment The Chromatrap® 96 DNA Micro Elution Purification Kit and its contents can be stored for up to 12 months after the date of receipt without showing any reduction in performance and quality.
Expiry Date 12 months