EasyScript Plus™ cDNA Synthesis Kit

Details for Product No. ABIN4219218, Supplier: Log in to see
Application
cDNA Synthesis (cDNA)
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Purpose cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization.
Brand EasyScript Plus™
Specificity - Maximal flexibility in priming - oligo(dT), random primers or gene-specific primers
- Robust cDNA synthesis from any RNA template
- High reproducibility and excellent yield
- This kit employs EasyScript™ Plus Reverse Transcriptase.
Characteristics cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization. Both random primers and oligo(dT) are included for a choice of general priming strategies and as alternatives to gene-specific primers.
Components cDNA Synthesis Kit contains all the materials required for first-strand cDNA synthesis, with the choice of using either Oligo (dT) and/or Random Primers. The Oligo (dT) anneals selectively to the poly (A) tail of mRNAs. Random Primers do not require the presence of poly (A) and can be used for the transcription of mRNA 5'-end regions.
Application Notes - Synthesizing cDNA from ssRNA
- DNA primer extension
- Sequencing dsDNA
- Constructing cDNA library
- Producing template for use in RT-PCR
- Labelling 3'-end of duplex DNA via end-filling reactions
- Generating probes for hybridization
Comment

100 x 20 μl reactions

Restrictions For Research Use only
Storage -20 °C
Storage Comment Store all components at -20°C.
Product cited in: Park, Ju, Park, Song, Shin, Lee, Jeong, Yu, Lee, Cho, Kim, Kim, Kim, Park, Chun: "PPARγ neddylation essential for adipogenesis is a potential target for treating obesity." in: Cell death and differentiation, Vol. 23, Issue 8, pp. 1296-311, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Amodio, Sasso, DAmbrosio, Scaloni, Moltedo, Franceschelli, Zambrano, Remondelli: "Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach." in: Cell biology and toxicology, Vol. 32, Issue 4, pp. 285-303, 2016 (PubMed).

Maher, Wojnarowicz, Ichu, Veldhoen, Lu, Lesperance, Propper, Helbing: "Rethinking the biological relationships of the thyroid hormones, l-thyroxine and 3,5,3'-triiodothyronine." in: Comparative biochemistry and physiology. Part D, Genomics & proteomics, Vol. 18, pp. 44-53, 2016 (PubMed).

Antony, Paillard, Moffat, Juskeviciute, Correnti, Bolon, Rubin, Csordás, Seifert, Hoek, Hajnóczky: "MICU1 regulation of mitochondrial Ca(2+) uptake dictates survival and tissue regeneration." in: Nature communications, Vol. 7, pp. 10955, 2016 (PubMed).

Juskeviciute, Dippold, Antony, Swarup, Vadigepalli, Hoek: "Inhibition of miR-21 rescues liver regeneration after partial hepatectomy in ethanol-fed rats." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 311, Issue 5, pp. G794-G806, 2016 (PubMed).

Kuttippurathu, Juskeviciute, Dippold, Hoek, Vadigepalli: "A novel comparative pattern analysis approach identifies chronic alcohol mediated dysregulation of transcriptomic dynamics during liver regeneration." in: BMC genomics, Vol. 17, pp. 260, 2016 (PubMed).