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EasyScript™ Two-Step RT-PCR Kit

Details for Product No. ABIN4219219, Supplier: Log in to see
Application
cDNA Synthesis (cDNA)
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Purpose Convenient and time-saving reaction set-up with EasyScript™ Two-Step RT-PCR Kit.
Brand EasyScript™
Specificity 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, abm offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. abm's MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.
- Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield and robust PCR performance
Characteristics cDNA Synthesis SuperMix provides highly efficient and specific conversion of RNA to cDNA, even from low-abundance transcripts. Furthermore, the unique blend of random primers and oligo(dT) primers in this SuperMix ensures optimal representation of all transcript sequences in the cDNA product. Overall, cDNA Synthesis SuperMix allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease.
Components This highly efficient two-step RT-PCR kit consists of two main components: EasyScript™ cDNA Synthesis SuperMix and TaqPlus 2X PCR MasterMix with dye.
Application Notes - Routine RT-PCR amplification of RNA templates
- Reliable RT-PCR based detection of gene expression
Comment

25 reactions
Two-Step RT-PCR kits provide all the necessary reagents required for reverse transcription and PCR amplification in two separate steps. The kit offers the flexibility of optimizing different RT-PCR applications with the choice of either EasyScriptTM or EasyScript PlusTM. The EasyScript PlusTM makes it possible to efficiently synthesize first-strand cDNA from RNA templates with secondary structures and high GC content.

Restrictions For Research Use only