5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit)

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Application
cDNA Synthesis (cDNA)
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Purpose 5X All-in-One RT MasterMix (with AccuRT Genomic DNA Removal Kit) offers a convenient and complete system to facilitate the complete elimination of contaminating genomic DNA in RNA preparations for rapid synthesis of high-quality cDNA for downstream applications.
Specificity - Efficient ds and ssDNA digestion in under 10 minutes
- Maximal RNA protection - No heating or organic extraction steps required
- Fewer handling errors, with only 1 liquid transfer step for reverse transcription
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent cDNA yield
Characteristics Removal of contaminating genomic DNA from your samples to reverse transcription of RNA in 30 minutes, with 5X All-In-One RT MasterMix (AccuRT Genomic DNA Removal Kit included).
Application Notes - Generation of templates for use in RT-PCR and qRT-PCR
- cDNA synthesis from ssRNA
- cDNA library construction
- Generation of probes for hybridization
- DNA primer extension
Comment

100 x 20 μl reactions
In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.

Restrictions For Research Use only
Storage -20 °C
Storage Comment Store all components at -20°C
Product cited in: Gao, Yang, Zhang, Chen, Tian: "Enhanced metabolic process to indole alkaloids in Clematis terniflora DC. after exposure to high level of UV-B irradiation followed by the dark." in: BMC plant biology, Vol. 16, Issue 1, pp. 231, 2016 (PubMed).

Smither, Pang, Brubaker: "Glucagon-Like Peptide-2 Requires a Full Complement of Bmi-1 for Its Proliferative Effects in the Murine Small Intestine." in: Endocrinology, Vol. 157, Issue 7, pp. 2660-70, 2016 (PubMed).