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ExCellenCT One-Step EvaGreen qRT-PCR-iCycler

Details for Product No. ABIN4219238, Supplier: Log in to see
Application
Quantitative real-time PCR (qPCR)
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Purpose Extracting and preparing RNA templates directly from cultured cells to be applied as template for One-Step qRT-PCR, with G471 One-Step EvaGreen qRT-PCR MasterMix.
Specificity The One-Step EvaGreen qRT-PCR system contains all necessary reagents for both reverse transcription and PCR amplification to occur in a single qPCR reaction tube, including a qRT-PCR Enzyme Mix and an EvaGreen qPCR MasterMix. a proprietary qRT-PCR Enzyme Mix contains stabilizers and enhancers to optimize the two reactions in a real-time "single step". Coupled together, this complete system provides the ultimate convenience in generating consistent, reproducible, and accurate results from 10-10^5 cells.
Characteristics ExCellenCT Lysis Kit is an unique and effective alternative method to provide RNA templates that are extracted and processed directly from cultured cells in less than 15 minutes, unveiling reverse-transcription-ready RNA/cell lysate from cultured cells without the time-consuming and hazardous-chemicals-involved RNA extraction and purification steps. RNA templates generated can then be directly used as template to be applied in One-Step qRT-PCR.
Application Notes Optimal working dilution should be determined by the investigator.
Comment

25 Preps, 100 x 20 μl reactions

Restrictions For Research Use only
Storage -20 °C
Storage Comment Stored all components at -20°C.