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Cloning Kits

Molecular cloning of single or multiple inserts into plasmids for various purposes is an essential part in any lab work ranging from expression of recombinant proteins to large scale sequencing projects. Traditionally, DNA pieces of interest are first excised using restriction endonucleases and then stitched together via compatible ends using a ligase. More recently, restriction free cloning has been complemented by techniques like Gibson cloning in which a virtually unlimited number of fragments can be concatenated through recombination. Cloning kits provide all the necessary reagents in one complete package.
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ISO 9001:2008

CloneEZ® PCR Cloning Kit is designed for quick and convenient PCR cloning.

Application:
Cloning
Components:
CloneEZ® Enzyme (5 U/µL): 50 µL
10X CloneEZ® Buffer: 100 µL
pUC57 Linearized with EcoRI/HindIII, positive control: 10 µL
1-kb Control Insert, positive control: 10 µL
Storage:
-20 °C
Publications
  • Wei, Oh, Million, Cate, Jin: "Simultaneous utilization of cellobiose, xylose, and acetic acid from lignocellulosic biomass for biofuel production by an engineered yeast platform." in: ACS synthetic biology, Vol. 4, Issue 6, pp. 707-13, 2015 (Pubmed)
  • Wang, Zhu, Chen, Wang: "mRNA m?A methylation downregulates adipogenesis in porcine adipocytes." in: Biochemical and biophysical research communications, Vol. 459, Issue 2, pp. 201-7, 2015 (Pubmed)
  • Zeng, Liu, Wang, Fang: "A bispecific antibody directly induces lymphoma cell death by simultaneously targeting CD20 and HLA-DR." in: Journal of cancer research and clinical oncology, Vol. 141, Issue 11, pp. 1899-907, 2015 (Pubmed)
  • Tang, Zhou, Zhang, Xiao, Hu, Zhang, Huang, Chen, Liu, Liang: "Synergetic action of domain II and IV underlies persistent current generation in Nav1.3 as revealed by a tarantula toxin." in: Scientific reports, Vol. 5, Issue , pp. 9241, 2015 (Pubmed)
  • Liu, Cui, Liu, Cui, Xia, Kobayashi, Zhou: "Enhancement of thermo-stability and product tolerance of Pseudomonas putida nitrile hydratase by fusing with self-assembling peptide." in: Journal of bioscience and bioengineering, Vol. 118, Issue 3, pp. 249-52, 2014 (Pubmed)
  • Hui, Al Zaki, Cheng, Popik, Zhang, Luning Prak, Tsourkas: "Facile method for the site-specific, covalent attachment of full-length IgG onto nanoparticles." in: Small (Weinheim an der Bergstrasse, Germany), Vol. 10, Issue 16, pp. 3354-63, 2014 (Pubmed)
  • Wang, Tan, Jiao, You, Zhang: "Analyzing cold tolerance mechanism in transgenic zebrafish (Danio rerio)." in: PLoS ONE, Vol. 9, Issue 7, pp. e102492, 2014 (Pubmed)
  • Elias, Crayton, Warden-Rothman, Tsourkas: "Quantitative comparison of tumor delivery for multiple targeted nanoparticles simultaneously by multiplex ICP-MS." in: Scientific reports, Vol. 4, Issue , pp. 5840, 2014 (Pubmed)
  • Wang, Yomano, Lee, York, Zheng, Mullinnix, Shanmugam, Ingram: "Engineering furfural tolerance in Escherichia coli improves the fermentation of lignocellulosic sugars into renewable chemicals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 10, pp. 4021-6, 2013 (Pubmed)
  • Kato, Aoyama, Maeshima: "The Ca(2+) -binding protein PCaP2 located on the plasma membrane is involved in root hair development as a possible signal transducer." in: The Plant journal : for cell and molecular biology, Vol. 74, Issue 4, pp. 690-700, 2013 (Pubmed)
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Catalog No. ABIN491483
$175.00
24 reactions
Plus shipping costs $45.00 Will be delivered in 4 to 6 Business Days
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The Ligation-Free Cloning kit is a versatile system to meet the demands of the routine and most challenging cloning projects.

Application:
Cloning
Catalog No. ABIN3188233
$143.61
25 reactions
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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T1 Cloning Kit is designed for cloning and sequencing Taq amplified PCR products.

Application:
Cloning
Catalog No. ABIN2952553
$200.00
Plus shipping costs $45.00 Will be delivered in 9 to 14 Business Days
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T1 Simple Cloning Vector eliminates the multi-cloning sites of T1 Cloning vector.

Application:
Cloning
Catalog No. ABIN2952554
$200.00
Plus shipping costs $45.00 Will be delivered in 11 to 16 Business Days
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An easy method for high-efficiency cloning of blunt-end PCR products.

Application:
Cloning
Catalog No. ABIN2952560
$212.50
Plus shipping costs $45.00 Will be delivered in 9 to 14 Business Days
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T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene.

Application:
Cloning
Catalog No. ABIN2952556
$212.50
Plus shipping costs $45.00 Will be delivered in 11 to 16 Business Days
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The Ligation-Free Cloning kit is a versatile system to meet the demands of the routine and most challenging cloning projects.

Application:
Cloning
Catalog No. ABIN3188234
$388.06
100 reactions
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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Blunt Simple Cloning Vector eliminates the multi-cloning sites of Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products. 

Application:
Cloning
Catalog No. ABIN2952558
$200.00
Plus shipping costs $45.00 Will be delivered in 9 to 14 Business Days
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Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products. 

Application:
Cloning
Catalog No. ABIN2952557
$200.00
Plus shipping costs $45.00 Will be delivered in 9 to 14 Business Days
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It is designed for cloning and sequencing blunt-end PCR products.

Application:
Cloning
Catalog No. ABIN2952559
$200.00
Plus shipping costs $45.00 Will be delivered in 9 to 14 Business Days
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T3 Cloning Vector provides dual-EcoR I and dual-Not I enzyme digestion sites. It is designed for cloning and sequencing Taq amplified PCR products.

Application:
Cloning
Catalog No. ABIN2952555
$200.00
Plus shipping costs $45.00 Will be delivered in 11 to 16 Business Days
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