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1. Prepare staining solution by diluting SafeView Plus™ 1:5,000 - 1:10,000 in TE, TAE or TBE buffer.2. After performing electrophoresis, place the dye-free gel in a plastic container and cover with the staining solution. Ensure the box is covered to protect the contents from light. Agitate gently and incubate at room temperature for 10 - 40 minutes.3. No destaining is required - visualize results directly under UV light.4. The staining solution may be resused up to 4 times. Keep stored at 4°C and protected from light. Do not store the solution in glass containers, as these surfaces adsorb the dye in the solution, reducing the staining efficacy