Safe ImageTM Basic DNA stain is used the same way as Ethidium Bromide in agarose gel electrophoresis. It is used directly in the gel and in the running buffer prior to the loading of the samples. The sensitivity range is between 0.1-0.3 ng. Under UV light, Safe ImageTM Basic DNA Stain emits green fluorescence when bound to dsDNA and ssDNA, and red fluorescence when bound to RNA. This stain has one excitation (490 nm) and two emission spectra (520 nm and 635 nm).