Safe Image™ Basic DNA Stain

Details for Product No. ABIN5684157,
Staining Methods (StM)
Purpose Safe Image™ Basic DNA Stain is used as a substitute for EtBr for post electrophoresis DNA staining.
Characteristics Safe ImageTM Stain represents a new and safe class of nucleic acid stains for the visualization of double-stranded DNA (dsDNA), singlestranded DNA (ssDNA), and RNA in agarose and polyacrylamide gels. The dyes have the capability to bind DNA and thus are developed to replace toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids. Safe Image™ products are non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests
Application Notes Optimal working dilution should be determined by the investigator.

Safe ImageTM Basic DNA stain is used the same way as Ethidium Bromide in agarose gel electrophoresis. It is used directly in the gel and in the running buffer prior to the loading of the samples. The sensitivity range is between 0.1-0.3 ng. Under UV light, Safe ImageTM Basic DNA Stain emits green fluorescence when bound to dsDNA and ssDNA, and red fluorescence when bound to RNA. This stain has one excitation (490 nm) and two emission spectra (520 nm and 635 nm).

Restrictions For Research Use only
Storage 4 °C
Storage Comment 4°C
Expiry Date 24 months