Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

368 Products
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    Enzyme
Gene
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Species
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Application
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Protein Type
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Format
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Supplier: Log in to see

TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for PCR provides all the necessary components for cDNA synthesis from total RNA or mRNA.

Application:
cDNA Synthesis
Components:
TransScript® All-in-One SuperMix for PCR, RNase-free Water
Storage:
-20 °C
Specificity:
One-tube format for simple and fast setup and reduce pipetting variability.
The optimal ratio of oligo(dT)18 primer to random primer(N9) for PCR-ready cDNA.
PCR-ready cDNA in 30 minutes (unsuitable for qPCR).
cDNA up to 12 kb.
Catalog No. ABIN5519497
$225.00
50 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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TransStart® FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 5X Pfu Buffer, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Unit Definition:
One unit of TransStart® FastPfu Fly DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
TransStart® FastPfu Fly DNA Polymerase has an extension rate of up to 6 kb/min. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.
Catalog No. ABIN5519533
$225.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Application:
DNA Modification
Concentration:
>2000 Kunitz U/mg
Catalog No. ABIN1543769
$226.92
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
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EvaGreen miRNA qPCR MasterMix is designed specifically for quantitative real-time analysis of miRNA samples.

Application:
Quantitative real-time PCR
Concentration:
2 x
Components:
Each EvaGreen 2X qPCR MasterMix is a 2X mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, reference dye and proprietary buffer components. Each set of EvaGreen qPCR MasterMix contains 4 vials, each containing 1.25 ml.
Storage:
-20 °C
Specificity:
The components of EvaGreen miRNA qPCR MasterMix have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hotstart Taq polymerase included in our MasterMix significantly reduces non-specific PCR amplification observed with regular Taq polymerase.
Catalog No. ABIN4219203
$228.09
4 x 1.25 mL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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EvaGreen miRNA qPCR MasterMix is designed specifically for quantitative real-time analysis of miRNA samples.

Application:
Quantitative real-time PCR
Concentration:
2 x
Storage:
-20 °C
Specificity:
The components of EvaGreen miRNA qPCR MasterMix have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hotstart Taq polymerase included in our MasterMix significantly reduces non-specific PCR amplification observed with regular Taq polymerase.
Catalog No. ABIN4219201
$228.09
4 x 1.25 mL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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EvaGreen miRNA qPCR MasterMix is designed specifically for quantitative real-time analysis of miRNA samples.

Application:
Quantitative real-time PCR
Concentration:
2 x
Components:
Each EvaGreen 2X qPCR MasterMix is a 2X mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, reference dye and proprietary buffer components. Each set of EvaGreen qPCR MasterMix contains 4 vials, each containing 1.25 ml.
Storage:
-20 °C
Specificity:
The components of EvaGreen miRNA qPCR MasterMix have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hotstart Taq polymerase included in our MasterMix significantly reduces non-specific PCR amplification observed with regular Taq polymerase.
Catalog No. ABIN4219204
$228.09
4 x 1.25 mL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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EvaGreen miRNA qPCR MasterMix is designed specifically for quantitative real-time analysis of miRNA samples.

Application:
Quantitative real-time PCR
Concentration:
2 x
Components:
Each EvaGreen 2X qPCR MasterMix is a 2X mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, reference dye and proprietary buffer components. Each set of EvaGreen qPCR MasterMix contains 4 vials, each containing 1.25 ml.
Storage:
-20 °C
Specificity:
The components of EvaGreen miRNA qPCR MasterMix have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hotstart Taq polymerase included in our MasterMix significantly reduces non-specific PCR amplification observed with regular Taq polymerase.
Catalog No. ABIN4219202
$228.09
4 x 1.25 mL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Catalyzes the ATP-dependent formation of a 3'→5' phosphodiester bond catalyzing intramolecular and intermolecular RNA strand joining

Catalog No. ABIN5684241
$229.00
100 μL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 2 to 3 Business Days
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2X PCR MasterMix with dye

Application:
Polymerase Chain Reaction
Storage:
4 °C,-20 °C
Catalog No. ABIN5684177
$229.00
200 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 2 to 3 Business Days
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2X PCR MasterMix

Application:
Polymerase Chain Reaction
Storage:
4 °C,-20 °C
Catalog No. ABIN5684176
$229.00
200 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 2 to 3 Business Days
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2X PCR MasterMix

Application:
Polymerase Chain Reaction
Catalog No. ABIN5684204
$229.00
200 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 2 to 3 Business Days
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Application:
DNA Modification
Concentration:
>500 U/mg
Catalog No. ABIN1543713
$236.15
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
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SuperMix with necessary reagents for cDNA synthesis and qPCR except total RNA/mRNA template and gene

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
5 minutes cDNA synthesis
cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519510
$237.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Streamline your PCR with 2X PCR MasterMix

Application:
Polymerase Chain Reaction
Concentration:
2 x
Components:
25 x 1.0 ml vials
Storage:
-20 °C
Specificity:
The use of Taq DNA Polymerase in this MasterMix offers:
- Robust PCR performance with great reproducibility
- High sensitivity
Catalog No. ABIN4219184
$241.03
25 mL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Streamline your PCR with 2X PCR MasterMix

Application:
Polymerase Chain Reaction
Concentration:
2 x
Components:
25 x 1.0 ml vials
Storage:
-20 °C
Specificity:
The use of Taq DNA Polymerase in this MasterMix offers:
- Robust PCR performance with great reproducibility
- High sensitivity
Catalog No. ABIN4219185
$241.03
25 mL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Bst DNA Polymerase, Large Fragment is a portion of DNA polymerase of Bacillus stearothermophilus, which catalyzes 5'→3' synthesis of DNA and strand displacement, but lacks 5'→3' and 3'→5' exonuclease activities.

Application:
Polymerase Chain Reaction
Components:
Bst DNA Polymerase, Large Fragment 8.0 unit/μl, 10x BstPol Reaction Buffer, 100 mM MgSO4
Unit Definition:
One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C.
Catalog No. ABIN5666436
$245.00
8000 U
Plus shipping costs $45.00
Will be delivered in 31 Business Days
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M-MLV Reverse Transcriptase (RNaseH-) is a recombinant reverse transcriptase expressed and purified from E. Coil. This enzyme can be used in cDNA synthesis with long messenger RNA templates due to the lacking of RNase H activity by point mutation.

Application:
cDNA Synthesis
Components:
M-MLV Reverse Transcriptase (RNaseH-)200U/μl, 5x M-MLV Reaction Buffer
Catalog No. ABIN5666451
$245.00
20000 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
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Extracting and preparing RNA templates directly from cultured cells to be reverse-transcribed into cDNA, with 5X All-In-One RT MasterMix.

Application:
Quantitative real-time PCR
Components:
5X All-In-One RT MasterMix
ExCellenCT Lysis Kit
Storage:
-20 °C
Specificity:
The 5X All-In-One RT MasterMix is a ready-to-use formulation of all the reagents necessary for first-strand cDNA synthesis, including proprietary EasyScriptTM Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, dNTPs, and a finely balanced ratio of Oligo (dT)s and Random Primers. Coupled together, this complete system provides the ultimate convenience in generating high-quality cDNA directly from 10-105 cultured cells, suitable for a wide range of downstream applications.
Catalog No. ABIN4219190
$247.50
100 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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One-Step first-strand cDNA synthesis with PCR

Application:
cDNA Synthesis
Components:
EasyScript® One-Step Enzyme Mix, 2x One-Step Reaction Mix, RNase-free Water
Storage:
-20 °C
Specificity:
Amplification of fragment up to 4 kb.
Catalog No. ABIN5519430
$250.00
200 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with blunt or cohesive end.

Application:
Ligation
Catalog No. ABIN2953733
$303.65
Plus shipping costs $45.00
Delivery in 11 to 16 Business Days