Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

286 Products
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Type
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    Enzyme
Gene
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Species
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Host
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Application
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Protein Type
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Ultimate sensitivity in real-time PCR with TaqProbe 2X qPCR MasterMix.

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
qPCR Instruments: Any qPCR instrument that supports multiplex reactions
Catalog No. ABIN4219208
$189.26
4 x 1.25 mL
Plus shipping costs $45.00
Will be delivered in 3 to 4 Business Days
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SuperMix with necessary reagents for cDNA synthesis and qPCR except total RNA/mRNA template and gene

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
5 minutes cDNA synthesis
cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519510
$197.00
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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Everything you need for PCR amplification and instant band visualization, in a single solution.

Application:
Polymerase Chain Reaction
Storage:
-20 °C
Specificity:
With Safe-Green™ 2X PCR Bestaq™ MasterMix, you can
- Reduce PCR set up time
- Eliminate ethidium bromide usage and associated waste disposal costs
- Increase PCR efficiency and reproducibility

The use of Bestaq™ DNA Polymerase in this MasterMix offers:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by up to 70 %
- Robust and high yield across a wide range of templates
- Efficient amplification of DNA templates up to 15kb
Catalog No. ABIN4219179
$202.21
5 mL
Plus shipping costs $45.00
Will be delivered in 3 to 4 Business Days
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cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA.

Application:
cDNA Synthesis
Components:
Contains: 50 reactions for 20 μL Systems
Storage:
-20 °C
Catalog No. ABIN2953677
$206.74
Plus shipping costs $45.00
Will be delivered in 11 to 16 Business Days
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SuperMix with all the necessary reagents for qPCR.

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
gDNA remover and 5xTransScript® All-in-One SuperMix for qPCR are provided for simultaneous gDNA removal and cDNA synthesis.
TransStart® Tip Green qPCR SuperMix is provided for qPCR.
5xTransScript® All-in-One No-RT Control SuperMix for qPCR is provided for experimental control.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519504
$262.00
1 kit
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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First-Strand cDNA Synthesis SuperMix for PCR

Application:
cDNA Synthesis
Components:
Contains: 50 reactions for 20 μL Systems
Storage:
-20 °C
Specificity:
First-Strand cDNA Synthesis SuperMix for PCR
Catalog No. ABIN2953681
$271.35
50 reactions
Plus shipping costs $45.00
Will be delivered in 11 to 16 Business Days
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This kit is suitable to generate qPCR-ready cDNA directly from cells.

Application:
cDNA Synthesis
Components:
C to C Lysis Buffer, Enzyme Mix, gDNA Remover, Reaction Mix, Oligo (dT)/RP Mix, RNase-free Water
Storage:
-20 °C
Catalog No. ABIN5519494
$308.00
25 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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SuperMix with necessary reagents for gDNA removal, cDNA synthesis and qPCR.

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
gDNA remover and 5xTransScript® II All-in-One SuperMix for qPCR are provided for simultaneous gDNA removal and cDNA synthesis.
TransStart® Tip Green qPCR SuperMix is provided for qPCR.
5xTransScript® II All-in-One No-RT Control SuperMix for qPCR is provided for experimental control.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519512
$415.00
1 kit
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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SuperMix with all the necessary components for miRNA detection

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
One-Step poly(A) tailing and cDNA synthesis
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519505
$492.00
1 kit
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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Application:
RNA Modification
Concentration:
>70 U/mg
Catalog No. ABIN1543661
$1,771.25
Plus shipping costs $45.00
Will be delivered in 2 to 3 Business Days
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The Uracil-DNA Glycocasylase (UDG) is expressed from recombinant clone of E. Coil, and the molecular weight is 25 kDa. The optimum reaction temperature of UDG is 50°C.

Storage:
-20 °C,-80 °C
Unit Definition:
One unit of the enzyme is the amount that completely cleaves 1 μg ds-DNA with dU in one hour at 37°C in the condition of 20 mM Tris-Hcl (PH8.0), 1 mM EDTA, 10 mM NaCl.
Catalog No. ABIN5666461
$50.00
500 U
Plus shipping costs $45.00
Will be delivered in 2 to 3 Business Days
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M-MLV Reverse Transcriptase (RNaseH-) is a recombinant reverse transcriptase expressed and purified from E. Coil. This enzyme can be used in cDNA synthesis with long messenger RNA templates due to the lacking of RNase H activity by point mutation.

Application:
cDNA Synthesis
Components:
M-MLV Reverse Transcriptase (RNaseH-)200U/μl, 5x M-MLV Reaction Buffer
Catalog No. ABIN5666450
$69.00
5000 U
Plus shipping costs $45.00
Will be delivered in 2 to 3 Business Days
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The Uracil-DNA Glycocasylase (UDG) is expressed from recombinant clone of E. Coil, and the molecular weight is 25 kDa. The optimum reaction temperature of UDG is 50°C.

Storage:
-20 °C,-80 °C
Unit Definition:
One unit of the enzyme is the amount that completely cleaves 1 μg ds-DNA with dU in one hour at 37°C in the condition of 20 mM Tris-Hcl (PH8.0), 1 mM EDTA, 10 mM NaCl.
Catalog No. ABIN5666462
$80.00
1000 U
Plus shipping costs $45.00
Will be delivered in 2 to 3 Business Days
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M-MLV Reverse Transcriptase (RNaseH-) is a recombinant reverse transcriptase expressed and purified from E. Coil. This enzyme can be used in cDNA synthesis with long messenger RNA templates due to the lacking of RNase H activity by point mutation.

Application:
cDNA Synthesis
Components:
M-MLV Reverse Transcriptase (RNaseH-)200U/μl, 5x M-MLV Reaction Buffer
Catalog No. ABIN5666451
$220.00
20000 U
Plus shipping costs $45.00
Will be delivered in 2 to 3 Business Days
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Concentration:
2 mg/mL
Components:
Protein supplied with 10X Reaction Buffer.
Storage:
-20 °C
Catalog No. ABIN3188204
$59.85
100 μL
Plus shipping costs $45.00
Will be delivered in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
XbaI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188218
$72.79
20000 U
Plus shipping costs $45.00
Will be delivered in 3 to 4 Business Days
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Application:
Polymerase Chain Reaction
Components:
- 0.1 U/µl Taq DNA Polymerase
- Reaction buffer
- 3 mM MgCl2
- 0.4 mM dNTPs
Storage:
-20 °C
Specificity:
The mixture is optimized for consistent and efficient routine PCR amplifications. It can amplify up to 8 kb fragment from lambda DNA.
Catalog No. ABIN1536552
$43.00
100 reactions
Plus shipping costs $45.00
Will be delivered in 6 to 8 Business Days
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Application:
Genome Editing with Engineered Nucleases
Concentration:
1000 nM
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188184
$92.21
50 μL
Plus shipping costs $45.00
Will be delivered in 3 to 4 Business Days
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Application:
Genome Editing with Engineered Nucleases
Concentration:
1000 nM
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188221
$92.21
50 μL
Plus shipping costs $45.00
Will be delivered in 3 to 4 Business Days
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Application:
Genome Editing with Engineered Nucleases
Concentration:
1000 nM
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188186
$92.21
50 μL
Plus shipping costs $45.00
Will be delivered in 3 to 4 Business Days