Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

359 Products
Data Quality
  • 18
  • 17
Type
  • 359
    Enzyme
Gene
  • 4
  • 2
  • 1
  • 1
Species
  • 8
  • 5
  • 2
  • 1
  • 1
Host
  • 11
Application
  • 128
  • 65
  • 38
  • 33
  • 32
Protein Type
  • 5
Format
  • 14
  • 1
Supplier: Log in to see

TransScript® Two-Step RT-PCR SuperMix performs first-strand cDNA synthesis and PCR in two steps.

Application:
cDNA Synthesis
Components:
5xTransScript® All-in-One SuperMix for PCR, 2xTransTaq® HiFi PCR SuperMix II, RNase-free Water
Storage:
-20 °C
Specificity:
Amplification of fragment up to 12 kb.
Catalog No. ABIN5519525
$215.00
1 kit
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
Supplier: Log in to see

Application:
DNA Amplification
Concentration:
10 U/μL
Components:
Enzyme supplied with 5X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.
Catalog No. ABIN3188235
$215.15
1000 U
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

Bst DNA Polymerase, Large Fragment is a portion of DNA polymerase of Bacillus stearothermophilus, which catalyzes 5'→3' synthesis of DNA and strand displacement, but lacks 5'→3' and 3'→5' exonuclease activities.

Application:
Polymerase Chain Reaction
Components:
Bst DNA Polymerase, Large Fragment 8.0 unit/μl, 10x BstPol Reaction Buffer, 100 mM MgSO4
Unit Definition:
One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C.
Catalog No. ABIN5666436
$220.00
8000 U
Plus shipping costs $45.00
Will be delivered in 31 Business Days
Supplier: Log in to see

M-MLV Reverse Transcriptase (RNaseH-) is a recombinant reverse transcriptase expressed and purified from E. Coil. This enzyme can be used in cDNA synthesis with long messenger RNA templates due to the lacking of RNase H activity by point mutation.

Application:
cDNA Synthesis
Components:
M-MLV Reverse Transcriptase (RNaseH-)200U/μl, 5x M-MLV Reaction Buffer
Catalog No. ABIN5666451
$220.00
20000 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
Supplier: Log in to see

Convenient and time-saving reaction set-up with cDNA Synthesis SuperMix.

Application:
cDNA Synthesis
Components:
cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
Storage:
-20 °C
Specificity:
- Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains EasyScript™ Plus Reverse Transcriptase.
Catalog No. ABIN4219172
$221.62
100 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

EvaGreen miRNA qPCR MasterMix is designed specifically for quantitative real-time analysis of miRNA samples.

Application:
Quantitative real-time PCR
Concentration:
2 x
Storage:
-20 °C
Specificity:
The components of EvaGreen miRNA qPCR MasterMix have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hotstart Taq polymerase included in our MasterMix significantly reduces non-specific PCR amplification observed with regular Taq polymerase.
Catalog No. ABIN4219201
$228.09
4 x 1.25 mL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

EvaGreen miRNA qPCR MasterMix is designed specifically for quantitative real-time analysis of miRNA samples.

Application:
Quantitative real-time PCR
Concentration:
2 x
Components:
Each EvaGreen 2X qPCR MasterMix is a 2X mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, reference dye and proprietary buffer components. Each set of EvaGreen qPCR MasterMix contains 4 vials, each containing 1.25 ml.
Storage:
-20 °C
Specificity:
The components of EvaGreen miRNA qPCR MasterMix have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hotstart Taq polymerase included in our MasterMix significantly reduces non-specific PCR amplification observed with regular Taq polymerase.
Catalog No. ABIN4219204
$228.09
4 x 1.25 mL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

EvaGreen miRNA qPCR MasterMix is designed specifically for quantitative real-time analysis of miRNA samples.

Application:
Quantitative real-time PCR
Concentration:
2 x
Components:
Each EvaGreen 2X qPCR MasterMix is a 2X mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, reference dye and proprietary buffer components. Each set of EvaGreen qPCR MasterMix contains 4 vials, each containing 1.25 ml.
Storage:
-20 °C
Specificity:
The components of EvaGreen miRNA qPCR MasterMix have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hotstart Taq polymerase included in our MasterMix significantly reduces non-specific PCR amplification observed with regular Taq polymerase.
Catalog No. ABIN4219203
$228.09
4 x 1.25 mL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

EvaGreen miRNA qPCR MasterMix is designed specifically for quantitative real-time analysis of miRNA samples.

Application:
Quantitative real-time PCR
Concentration:
2 x
Components:
Each EvaGreen 2X qPCR MasterMix is a 2X mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, reference dye and proprietary buffer components. Each set of EvaGreen qPCR MasterMix contains 4 vials, each containing 1.25 ml.
Storage:
-20 °C
Specificity:
The components of EvaGreen miRNA qPCR MasterMix have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hotstart Taq polymerase included in our MasterMix significantly reduces non-specific PCR amplification observed with regular Taq polymerase.
Catalog No. ABIN4219202
$228.09
4 x 1.25 mL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

TransScript® II Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity and increased thermostability.

Application:
cDNA Synthesis
Components:
TransScript® II Reverse Transcriptase, 5xTS II RT Buffer, Anchored Oligo (dT)20 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of TransScript® II RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Increased thermostability for more full-length cDNA products.
Reaction temperature at 42 °C-55 °C.
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
cDNA up to 15 kb.
Catalog No. ABIN5519516
$231.00
10000 units
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
Supplier: Log in to see

Reverse Transcriptases

Components:
EvoTM Reverse Transcriptase (200 U/μl), 5X RT buffer
Storage:
-20 °C
Catalog No. ABIN5684183
$236.00
100 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 2 to 3 Business Days
Supplier: Log in to see

2X PCR MasterMix

Application:
Polymerase Chain Reaction
Storage:
4 °C,-20 °C
Catalog No. ABIN5684203
$236.00
200 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 2 to 3 Business Days
Supplier: Log in to see

NovoTM Reverse Transcriptase is the ultimate solution to synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content).

Components:
NovoTM Reverse Transcriptase (200 U/μl), 5X RT Buffer
Storage:
-20 °C
Catalog No. ABIN5684225
$236.00
25 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 2 to 3 Business Days
Supplier: Log in to see

Ensures complete DNA removal from RNA extracted using RNA extraction kits for use in DNA sensitive downstream applications

Catalog No. ABIN5684226
$236.00
100 μL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 2 to 3 Business Days
Supplier: Log in to see

2X PCR MasterMix

Application:
Polymerase Chain Reaction
Storage:
4 °C,-20 °C
Catalog No. ABIN5684233
$236.00
200 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 2 to 3 Business Days
Supplier: Log in to see

2X PCR MasterMix with dye

Application:
Polymerase Chain Reaction
Storage:
4 °C,-20 °C
Catalog No. ABIN5684234
$236.00
200 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 2 to 3 Business Days
Supplier: Log in to see

2X PCR MasterMix, Whole Blood PCR Mix

Application:
Polymerase Chain Reaction
Catalog No. ABIN5684249
$236.00
200 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 2 to 3 Business Days
Supplier: Log in to see

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Application:
Ligation
Components:
T4 DNA Ligase 250 unit/μl, 10x T4 DNA Ligase Buffer
Unit Definition:
One unit of T4 DNA Ligase is defined as the amount of enzyme required to catalyze the ligation of greater than 90% of λ DNA-HindIII fragments in 20 μl at 16°C in 30 minutes. One unit of this enzyme corresponds to 0.008 Weiss units.
Catalog No. ABIN5666455
$240.00
5 x 25000 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
Supplier: Log in to see

Streamline your PCR with 2X PCR MasterMix

Application:
Polymerase Chain Reaction
Concentration:
2 x
Components:
25 x 1.0 ml vials
Storage:
-20 °C
Specificity:
The use of Taq DNA Polymerase in this MasterMix offers:
- Robust PCR performance with great reproducibility
- High sensitivity
Catalog No. ABIN4219184
$241.03
25 mL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

Streamline your PCR with 2X PCR MasterMix

Application:
Polymerase Chain Reaction
Concentration:
2 x
Components:
25 x 1.0 ml vials
Storage:
-20 °C
Specificity:
The use of Taq DNA Polymerase in this MasterMix offers:
- Robust PCR performance with great reproducibility
- High sensitivity
Catalog No. ABIN4219185
$241.03
25 mL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days