Taq DNA Polymerase

Details for Product No. ABIN1536547,
Thermus aquaticus
Polymerase Chain Reaction (PCR)
Characteristics Taq DNA Polymerase is a thermostable DNA Polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used for PCR.
Application Notes The applications of Taq DNA Polymerase include the following: PCR 3' A-tailing of blunt ends Primer extension DNA sequencing.

Terminal transferase activity: Taq DNA Polymerase has terminal transferase activity, which results in the addition of a single nucleotide (adenosine) at the 3' end of the extension product.
High purity: No contamination activity has been detected in standard test reactions.
Terminal Transferase Activity: A single nucleotide (adenosine) is added to the 3' end of the extension product.
High-purity: No contamination activity has been detected in standard test reactions.
Unit Definition: one unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

Restrictions For Research Use only
Format Liquid
Buffer 500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs. Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity. Concentration: Taq is delivered in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.
Storage -20 °C
Storage Comment Store the product at -20°C. The enzyme can be shipped at room temperature or even 37°C for seven days without any loss of activity.
Image no. 1 for Taq DNA Polymerase (ABIN1536547) Taq DNA Polymerase
Product cited in: Lee, Rollwagen-Bollens, Bollens, Faber-Hammond: "Environmental influence on cyanobacteria abundance and microcystin toxin production in a shallow temperate lake." in: Ecotoxicology and environmental safety, Vol. 114, pp. 318-25, 2015 (PubMed).

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Frías, Brito, González, González: "The phytotoxic activity of the cerato-platanin BcSpl1 resides in a two-peptide motif on the protein surface." in: Molecular plant pathology, Vol. 15, Issue 4, pp. 342-51, 2014 (PubMed).

Acanda, Martínez, Prado, González, Rey: "EMS mutagenesis and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of grapevine." in: Plant cell reports, Vol. 33, Issue 3, pp. 471-81, 2014 (PubMed).

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Firlej, Doyon, Harwood, Brodeur: "A multi-approach study to delineate interactions between carabid beetles and soybean aphids." in: Environmental entomology, Vol. 42, Issue 1, pp. 89-96, 2013 (PubMed).

Gandhirajan, Meng, Chandramoorthy, Mallilankaraman, Mancarella, Gao, Razmpour, Yang, Houser, Chen, Koch, Wang, Soboloff, Gill, Madesh: "Blockade of NOX2 and STIM1 signaling limits lipopolysaccharide-induced vascular inflammation." in: The Journal of clinical investigation, Vol. 123, Issue 2, pp. 887-902, 2013 (PubMed).

Wang, Kohalmi, Svircev, Wang, Sanfaçon, Tian: "Silencing of the host factor eIF(iso)4E gene confers plum pox virus resistance in plum." in: PLoS ONE, Vol. 8, Issue 1, pp. e50627, 2013 (PubMed).