Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

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Golden qPCR SYBR Green Master Mix(2x) is an optimal 2x concentrated solution of hot-start DNA polymerase, MgCl2, dNTPs, SYBR Green I dye and all other components required for quantitative real-time PCR, except DNA template and primers. This is a premixed, ready-to-use solution with high sensitivity and specificity.

Application:
Quantitative real-time PCR
Storage:
4 °C,-20 °C
Catalog No. ABIN5666447
€459.29
25 mL
Plus shipping costs €45.00
Delivery in 2 to 3 Business Days
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All-in-One First-Strand cDNA Synthesis SuperMix for qPCR

Application:
cDNA Synthesis
Concentration:
5 x
Components:
Contains: 50 reactions for 20 μL Systems
Storage:
-20 °C
Catalog No. ABIN2953687
€539.33
50 reactions
Plus shipping costs €45.00
Delivery in 11 to 16 Business Days
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A template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules.

Catalog No. ABIN5684247
€552.21
2500 U
Plus shipping costs €45.00
Delivery in 2 to 3 Business Days
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Sortase refers to a group of prokaryotic transpeptidase that attaches surface proteins covalently to the cell wall existing in almost all Gram-positive bacteria. It contains two sub-groups, Sortase A and Sortase B.

Catalog No. ABIN5666453
€575.22
100 μg
Plus shipping costs €45.00
Delivery in 2 to 3 Business Days
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UNG efficiently hydrolyzes the release of free uracil from uracil-containing single stranded or double stranded DNA, but not from oligomers (6 or less bases).

Application:
Quantitative real-time PCR
Concentration:
5000 units/mL
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double stranded, uracil containing DNA.
Catalog No. ABIN3188315
€705.00
1500 U
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 6 to 7 Business Days
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Taq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.

Application:
Polymerase Chain Reaction
Components:
Taq DNA Polymerase 5 U/μl, 10xTaq PCR Buffer, 25 mM MgCl2
Unit Definition:
One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 75°C.
Catalog No. ABIN5666458
€796.46
5000 U
Plus shipping costs €45.00
Delivery in 2 to 3 Business Days
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Application:
RNA Modification
Concentration:
>70 U/mg
Catalog No. ABIN1543661
€1,436.35
5 g
Plus shipping costs €45.00
Delivery in 2 to 3 Business Days
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Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a 5´ flap endonuclease activity.

Application:
Polymerase Chain Reaction
Unit Definition:
One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 75°C.
Catalog No. ABIN5666443
€1,460.18
5000 U
Plus shipping costs €45.00
Delivery in 2 to 3 Business Days