TurboNuclease

Details for Product No. ABIN2506908, Supplier: Log in to see
Reactivity
Serratia marcescens
Host
Escherichia coli (E. coli)
Application
DNA Modification (DNA Mod)
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Purpose Pure recombinant active bacterial TurboNuclease
Specificity A nonspecific endonuclease
Characteristics TurboNuclease is a recombinant form of Serratia macescens extracellular endonuclease (encoded by the same gene of Benzonase) produced in E. coli using a proprietary process. This nonspecific endonuclease hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5'-phosphorylated oligonucleotides of 1-4 bases in length. TurboNuclease is a highly purified homodimer of 27 kDa subunits that has exceptional high specific activity and is free of protease activity. It is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.
Alternate Names: TurboNuclease
Endotoxin Level < 0.25 EU / 1,000 units of TurboNuclease as determined by the LAL Gel-Clot Assay.
Biological Activity Comment >1.3x10⁶ units/mg
Unit Definition One unit of TurboNuclease converts 50 μg of salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37°C in a reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl₂.
Molecular Weight 27.0 kDa
Application Notes TurboNuclease is very effective in reducing the viscosity of cell lysates. It removes nucleic acid contamination from sample preparations such as Adenovirus and AAV purification. It reduces smearing when used with 10% SDS to make whole cell lysate for SDSPAGE. It may reduce or prevent clumping of concentrated cells and frozen cells following thawing. It also replaces crude DNase I in many applications. To reduce viscosity of cell lysate, 10-100 units of TurboNuclease can be used for each gram of cell paste. The efficiency of viscosity reduction may vary with buffers, cell types, and cell lysis methods used. Due to its high specificity, the total amount of TurboNuclease added is less than 1 µg/ml of lysate and will not complicate any downstream process.
Restrictions For Research Use only
Format Liquid
Concentration 250 U/μL
Buffer 250 units/μl in a storage buffer of 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgCl₂ and 50 % glycerol
Handling Advice Centrifuge the vial prior to opening.
Storage -20 °C
Storage Comment Store the product at -20°C. TurboNuclease is stable in the storage buffer at 37°C for at least three weeks without any loss of activity.
Expiry Date 12 months
Supplier Images
Polymerase Chain Reaction (PCR) image for TurboNuclease (ABIN2506908) TurboNuclease
SDS-PAGE (SDS) image for TurboNuclease (ABIN2506908) TurboNuclease (Image 2)