PvuRts1 I restriction enzyme

Details for Product No. ABIN2669403,
Reactivity
Proteus vulgaris
Host
Escherichia coli (E. coli)
Application
Restriction Digest (RD)
Options
Specificity PvuRts1 I restriction enzyme recognizes the sequence hmCN11-12/N9-10G.
Components 50 Units PvuRts1 I restriction enzyme
10X PvuRts1 I Reaction Buffer
1 M DTT
Application Notes PvuRts1 I restriction enzyme can be used to directly cleave hydroxymethylated DNA in its non-glucosylated form. The PvuRts1 I enzyme also digests α and β-glucosylated hydroxymethylcytosine DNA, but at a lower efficiency than the non-glucosylated form. The enzyme is specific to 5-hmC DNA and will not digest 5-methylcytosine residues or unmethylated DNA. The enzyme is best suited for use in regions of high 5-hmC density, such as gene bodies.
Comment

One unit is defined as the amount of enzyme required to fully cleave 1 µg of 5-hmC DNA Standard (ABIN2866136) in 30 minutes at 22°C in a total reaction volume of 30 µl. A pilot titration of enzyme is recommended for cleavage of genomic DNA.

Restrictions For Research Use only
Storage -20 °C/-80 °C
Storage Comment The PvuRts1 I enzyme is supplied in 50% glycerol and can be stored at -20°C. Make small aliquots and store at -80°C for prolonged storage.
Supplier Images
Restriction Digest (RD) image for PvuRts1 I restriction enzyme (ABIN2669403) Figure 1: One µg of unmethylated (Un), 5-methylcytosine (mC) or 5-hydroxymethylcytosi...