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Nt.BstNBI (Bst Nickase) Enzyme

DNA Mod 10 U/μL
Catalog No. ABIN3188203
$92.21
Plus shipping costs $45.00 and $20.00 dry ice
1000 U
local_shipping Shipping to: United States
Delivery in 11 to 21 Business Days
  • Application
    DNA Modification (DNA Mod)
    Characteristics
    Nt.BstNBI (Bst Nickase) is a nicking endonuclease from Bacillus stearothermophilus that cleaves one DNA strand of a double-stranded DNA substrate. This site-specific endonuclease cleaves a single strand of DNA four base pairs away and 3' of its recognition sequence.
    Components
    Enzyme supplied with 10X Reaction Buffer
    Unit Definition
    One unit is defined as the amount of Nt.BstNBI (Bst Nickase) that digests 1 μg of T7 DNA into 5 distinct bands (13, 9, 7, 6, and 4 kb) in 1 hour at 55°C in a reaction volume of 50 μl.
  • Comment

    • Single strand DNA nicking.

    Restrictions
    For Research Use only
  • Concentration
    10 U/μL
    Buffer
    10 mM Tris-HCl ( pH 7.5), 0.1 mM EDTA, 1 mM DTT, 50 mM KCl, 200 μg/mL BSA and 50 % (v/v) Glycerol.
    Storage
    -20 °C
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