RNase H (Ribonuclease H), E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA strands in RNA-DNA hybrids. This enzyme cleaves the 3'-O-P-bond of RNA and generates 3' hydroxyl and 5' phosphate products. RNase H does not digest single-stranded or double-stranded DNA and RNA.
Enzyme supplied with 10X Reaction Buffer
One unit is defined as the amount of RNase H, E. coli that is required to hydrolyze 1 nmol of the RNA in radiolabeled poly(rA):poly(dT), to acid-soluble ribonucleotides in a total reaction volume of 50 μl in 20 minutes at 37°C in 1X RNase H, E. coli Reaction Buffer with 10 nmol radiolabeled poly(rA) and 12.5 μg poly(dT).