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T7 Endonuclease I Enzyme

DNA Mod 10 U/μL
Catalog No. ABIN3188214
$189.26
Plus shipping costs $45.00 and $22.00 dry ice
1000 U
local_shipping Shipping to: United States
Delivery in 11 to 21 Business Days
  • Application
    DNA Modification (DNA Mod)
    Characteristics
    T7 Endonuclease I recognizes and cleaves mismatched DNA, heteroduplex DNA, cruciform DNA, Holliday structures and/or junctions, as well as nicked double-stranded DNA. This enzyme has a preference for single stranded over double stranded DNA with cleavage occurring at the first, second or third phosphodiester bond that is 5´ to the mismatch.
    Components
    Enzyme supplied with 10X Reaction Buffer
    Unit Definition
    One unit is defined as the amount of T7 Endonuclease I that linearizes over 90% of 1 μg of supercoiled cruciform DNA in a 50 μl volume in 1 hour at 37°C.
  • Comment

    • Mutation detection assay
    • Detect and cleave heteroduplex and/or nicked DNA
    • Cleave DNA randomly for use in shotgun cloning

    Restrictions
    For Research Use only
  • Concentration
    10 U/μL
    Buffer
    25 mM Tris-HCl ( pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.15 % Triton® X-100 and 50 % (v/v) Glycerol.
    Storage
    -20 °C
    Storage Comment
    Store all components at -20°C. Avoid repeated freeze-thaw cycles of all components to retain maximum performance. All components are stable for 1 year from the date of shipping when stored and handled properly.
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