XbaI

Details for Product No. ABIN3188218,
Application
Restriction Digest (RD)
Options
Components XbaI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Comment

  • Restriction enzyme digestion of doublestranded DNA
  • Reaction: 1X Universal Restriction Enzyme Reaction Buffer. Incubate at 37°C
  • Source:An E. coli strain that carries the XbaI gene from Xanthomonas badrii (ATCC 11672). The XbaI enzyme recognizes and cuts the sequence T^CTAGA.
  • Reaction Buffer: Buffer 1: 10%, Buffer 2: 100%, Buffer 3: 75%, Universal Buffer: 100%
  • Methylation Sensitivity
  • dam methylation: Blocked by Overlapping
  • dcm methylation: Not Sensitive
  • CpG Methylation: Not Sensitive
  • Heat Inactivation: 65 °C for 20 min

Restrictions For Research Use only
Concentration 20000 U/mL
Buffer 10 mM Tris-HCl ( pH 7.5), 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 50 % (v/v) Glycerol
Storage -20 °C