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T4 DNA Ligase Enzyme

Lig 5 U/μL
Catalog No. ABIN411759
$150.00
Plus shipping costs $45.00
250 U
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
  • Host
    Escherichia coli (E. coli)
    Application
    Ligation (Lig)
    Purpose
    T4 DNA Ligase (5 u/ul)
    Specificity
    An enzyme which anneals DNA strands together
    Characteristics
    T4 DNA ligase catalyzes the formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxy ends of double-stranded DNA. Single stranded nicks in double-stranded DNA are also closed by T4 DNA ligase. T4 DNA ligase needs ATP as co-factor.
    Alternate Names: T4 DNA Ligase
  • Comment

    Unit Definition: One unit of T4 DNA ligase is the amount of enzyme activity required to ligate EcoR I digested 1.0 μg of plasmid DNA in 5 minutes at 20°C in a 20 μl Reaction.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    5 U/μL
    Buffer
    ENZYME STORAGE BUFFER: 20 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.1 mM EDTA, 5 mM DTT, 50 % glycerol (v/v).
    Handling Advice
    Centrifuge the vial prior to opening.
    Storage
    -80 °C
    Storage Comment
    -80°C
    Expiry Date
    12 months
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