2X PCR Bestaq™ MasterMix with dye

Details for Product No. ABIN4219176,
Polymerase Chain Reaction (PCR)
Purpose Streamline your PCR with 2X PCR MasterMix
Brand Bestaq™
Specificity The ready-to-use PCR MasterMix is a proprietary mixture of high quality DNA Polymerase, dNTPs, Mg2+, and PCR buffer in a 2X concentration. It contains all necessary reagents for DNA amplification. To set up a PCR, simply add DNA template, primers, and nuclease-free H2O to yield a 1X reaction mix. This PCR MasterMix contains loading dye. It allows direct gel electrophoresis of PCR products without the need of adding loading dye to finished PCR products.
The use of Bestaq™ DNA Polymerase in this MasterMix offers:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by up to 70 %
- Robust and high yield across a wide range of templates
- Efficient amplification of DNA templates up to 15kb
Characteristics 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, we offer a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. The MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.
Application Notes - High-throughput PCR
- Robust amplification of AT- and GC-Rich sequences
- NGS Library construction

All PCR experiments should be assembled in a nuclease-free environment. In addition, DNA sample preparation, reaction set-up, and subsequent reaction(s) should be performed in separate areas to avoid cross contamination. The use of "clean" pipettors designated for PCR and aerosol resistant barrier tips are recommended. A negative control reaction (omitting DNA template) should be performed in tandem with sample PCR to confirm the absence of DNA contamination.
1. Add the following components into a sterile 0.2 ml PCR tube on ice (50 μlreaction volume).
- 2X PCR MasterMix 25 μl (Final Concentration 1X)
- DNA Template ~100 ng (Final Concentration ~2 ng/μl)
- Forward Primer (10 μM) 1-2.5 μl (Final Concentration 200-500 nM)
- Reverse Primer (10 μM) 1-2.5 μl (Final Concentration 200-500 nM)
- Nuclease-free H2O Up to 50 μl
2. Mix tube contents carefully and thoroughly, then collect all reagents by a brief centrifugation.
3. Choose an appropriate PCR amplification protocol for different DNA Polymerases.
4. Final PCR products are analyzed by agarose gel electrophoresis

Restrictions For Research Use only
Concentration 2 x
Storage 4 °C/-20 °C
Storage Comment Store at -20°C for long-term storage and at 4°C for up to two weeks.