5X All-In-One RT MasterMix

Details for Product No. ABIN4219182,
cDNA Synthesis (cDNA)
Purpose From RNA template to cDNA in just 15 minutes completely hassle-free.
Specificity - Reduction in handling errors with only 1 liquid transfer step
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent yield
Characteristics With 5X All-In-One RT MasterMix, just add RNA and this optimized system will provide sensitive and reliable cDNA synthesis over a dynamic range of input RNA. The use of this MasterMix eliminates multiple component additions providing exceptional reproducibility and precision, in addition to offering the end-user ultimate convenience.
Application Notes - Generation of templates for use in RT-PCR and qRT-PCR
- cDNA synthesis from ssRNA
- cDNA library construction
- Generation of probes for hybridization
- DNA primer extension

200 x 10 μl reactions
In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.

Restrictions For Research Use only
Storage -20 °C
Storage Comment Store all components at -20°C.
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