EvaGreen 2X qPCR MasterMix-iCycler

Details for Product No. ABIN4219195,
Quantitative real-time PCR (qPCR)
Purpose Guaranteed high-performance real-time PCR using EvaGreen 2X qPCR MasterMix.
Specificity QPCR Instruments:
- BioRad® iCycler®
- iQ™5
- MyiQ™
Characteristics - Enable streamlined protocol in a simple reaction set-up
- Allow accurate quantification of a variety of gene targets
- Reduce pipetting steps to minimize the risk of contamination
- Compatible with most real-time PCR instruments
Components Each EvaGreen 2X qPCR MasterMix is a 2X mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, reference dye and proprietary buffer components. Each set of EvaGreen qPCR MasterMix contains 4 vials, each containing 1.25 ml.
Application Notes - Gene-expression analysis
- Gene knockdown validation
- Microarray validation
- High throughput applications
- Virus detection and quantification

4 X 1.25 ml, for 500 reactions (20 μl)
1. Aliquot reagents to avoid contamination and to avoid repeated freeze-thaw cycles.
2. Primer concentration should not be high, a concentration of 100 nM to 300 nM of each primer usually gives the best results.
3. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: preparing amplicon specific pre-mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range.
4. For optimal results, it is recommended that the primers are 18-22 nucleotides in length with a Tm of 58°C-60°C and the size of target is about 100-250 bp.

Restrictions For Research Use only
Storage -20 °C
Storage Comment Store at -20°C and protect from light. Store at 4°C if the MasterMix will be used within 2 weeks.
Product cited in: Chi, Bhagwat, Tang, Xiang: "Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs." in: Methods in molecular biology (Clifton, N.J.), Vol. 1405, pp. 163-78, 2016 (PubMed).

Lee, Lee, Shim: "Emerin suppresses Notch signaling by restricting the Notch intracellular domain to the nuclear membrane." in: Biochimica et biophysica acta, Vol. 1864, Issue 2, pp. 303-313, 2016 (PubMed).

Gao, Yang, Zhang, Chen, Tian: "Enhanced metabolic process to indole alkaloids in Clematis terniflora DC. after exposure to high level of UV-B irradiation followed by the dark." in: BMC plant biology, Vol. 16, Issue 1, pp. 231, 2016 (PubMed).