EvaGreen 2X qPCR MasterMix-No Dye

Details for Product No. ABIN4219211,
Quantitative real-time PCR (qPCR)
Purpose Guaranteed high-performance real-time PCR using EvaGreen 2X qPCR MasterMix.
Specificity QPCR Instruments:
- BioRad® CFX96
- CFX384
- Chromo4™
- CFX Connect™
- Opticon 2
- MiniOpticon™
- Roche LightCycler® (2.0, 1.5, 480, 1536, Nano)
- MJ Research Opticon™
- Opticon™ 2
- Chromo® 4
- Eppendorf® Realplex 4
- BioGene SynChron™
- Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600)
- Eppendorf Mastercycler® realplex (s, 4 , 4s)
- Pro (S, 384)
- Nexus (gradient, eco, flat)
- Cepheid SmartCycler®
- GeneXpert
- Enigma® ML
- Idaho LightScanner® (24, 32)
- RapidCycler®2
- R.A.P.I.D (LT, LT Food)
- Qiagen Rotor-Gene™ (Q, 6000)
- Takara Dice™
-T hermo Scientific PikoReal
- DNA-Technology DT96
- DTlite
- DT-322
- Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)
- Bioneer ExicyclerTM
Characteristics - Enable streamlined protocol in a simple reaction set-up
- Allow accurate quantification of a variety of gene targets
- Reduce pipetting steps to minimize the risk of contamination
- Compatible with most real-time PCR instruments
Components Each EvaGreen 2X qPCR MasterMix is a 2X mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, reference dye and proprietary buffer components. Each set of EvaGreen qPCR MasterMix contains 4 vials, each containing 1.25 ml.
Application Notes - Gene-expression analysis
- Gene knockdown validation
- Microarray validation
- High throughput applications
- Virus detection and quantification

4 X 1.25 ml, for 500 reactions (20 μl)
1. Aliquot reagents to avoid contamination and to avoid repeated freeze-thaw cycles.
2. Primer concentration should not be high, a concentration of 100 nM to 300 nM of each primer usually gives the best results.
3. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: preparing amplicon specific pre-mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range.
4. For optimal results, it is recommended that the primers are 18-22 nucleotides in length with a Tm of 58°C-60°C and the size of target is about 100-250 bp.

Restrictions For Research Use only
Storage -20 °C
Storage Comment Store at -20°C and protect from light. Store at 4°C if the MasterMix will be used within 2 weeks.
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Hanson, Hamilton, Perlman: "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." in: BMC evolutionary biology, Vol. 16, Issue 1, pp. 228, 2016 (PubMed).

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