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EasyPfu DNA Polymerase Enzyme

PCR
Catalog No. ABIN5519422
$75.79
Plus shipping costs $45.00 and $22.00 dry ice
local_shipping Shipping to: United States
Delivery in 12 to 16 Business Days
  • Application
    Polymerase Chain Reaction (PCR)
    Purpose
    EasyPfu DNA Polymerase is an engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity.
    Brand
    EasyPfu
    Specificity
    EasyPfu DNAPolymerase possesses a proofreading 3'-5' exonuclease activity.
    Characteristics
    - EasyPfu DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
    - Extension rate is about 0.5 kb/min.
    - PCR products can be directly cloned into pEASY®-Blunt vectors.
    - Amplification of genomic DNA fragment up to 6 kb.
    - Amplification of plasmid DNA fragment up to 10 kb.
    Components
    DNA Polymerase, 10X Taq Buffer, 6X DNA Loading Buffer, 50 mM MgSO4
    Unit Definition
    One unit of EasyPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
  • Application Notes
    High fidelity PCR, Blunt-end cloning, Site-directed mutagenesis
    Comment

    EasyPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of EasyPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

    Restrictions
    For Research Use only
  • Buffer
    Storage Buffer:50 mM Tris-HCl ( pH 8.0), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50 % (v/v) glycerol stabilizers
    10xEasyPfu Buffer with 20 mM MgSO4: 200 mM Tris-HCl ( pH 8.8), 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, others
    Storage
    -20 °C
    Storage Comment
    at -20°C for two years
    Expiry Date
    24 months
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