EasyPfu DNA Polymerase
PCR
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- Application
- Polymerase Chain Reaction (PCR)
- Purpose
- EasyPfu DNA Polymerase is an engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity.
- Brand
- EasyPfu
- Specificity
- EasyPfu DNAPolymerase possesses a proofreading 3'-5' exonuclease activity.
- Characteristics
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- EasyPfu DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
- Extension rate is about 0.5 kb/min.
- PCR products can be directly cloned into pEASY®-Blunt vectors.
- Amplification of genomic DNA fragment up to 6 kb.
- Amplification of plasmid DNA fragment up to 10 kb. - Components
- DNA Polymerase, 10X Taq Buffer, 6X DNA Loading Buffer, 50 mM MgSO4
- Unit Definition
- One unit of EasyPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
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- Application Notes
- High fidelity PCR, Blunt-end cloning, Site-directed mutagenesis
- Comment
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EasyPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of EasyPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.
- Restrictions
- For Research Use only
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- Buffer
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Storage Buffer:50 mM Tris-HCl ( pH 8.0), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50 % (v/v) glycerol stabilizers
10xEasyPfu Buffer with 20 mM MgSO4: 200 mM Tris-HCl ( pH 8.8), 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, others - Storage
- -20 °C
- Storage Comment
- at -20°C for two years
- Expiry Date
- 24 months
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