EasyPfu DNA Polymerase (with 2.5 mM dNTPs)

Details for Product No. ABIN5519425,
Polymerase Chain Reaction (PCR)
Purpose EasyPfu DNA Polymerase is an engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity.
Brand EasyPfu
Specificity EasyPfu DNAPolymerase possesses a proofreading 3'-5' exonuclease activity.
Characteristics - EasyPfu DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
- Extension rate is about 0.5 kb/min.
- PCR products can be directly cloned into pEASY®-Blunt vectors.
- Amplification of genomic DNA fragment up to 6 kb.
- Amplification of plasmid DNA fragment up to 10 kb.
Components DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer, 50 mM MgSO4
Unit Definition One unit of EasyPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Application Notes High fidelity PCR, Blunt-end cloning, Site-directed mutagenesis

EasyPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of EasyPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

Restrictions For Research Use only
Buffer Storage Buffer:50 mM Tris-HCl ( pH 8.0), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50 % (v/v) glycerol stabilizers
10xEasyPfu Buffer with 20 mM MgSO4: 200 mM Tris-HCl ( pH 8.8), 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, others
Storage -20 °C
Storage Comment at -20°C for two years
Expiry Date 24 months