EasyScript® Reverse Transcriptase

Details for Product No. ABIN5519431,
cDNA Synthesis (cDNA)
Purpose EasyScript® Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity.
Brand EasyScript®
Specificity Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 8 kb.
Characteristics The enzyme is purified to near homogeneity from E.coli containing the modified M-MLV RT gene.
Components EasyScript® Reverse Transcriptase, 5xES RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Unit Definition One unit of EasyScript® RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37oC using Poly(A)/Oligo(dT) as template/primer.
Application Notes First-strand cDNA synthesis, Multiple copy gene detection
Restrictions For Research Use only
Buffer 5xES RT Buffer: 375 mM KCl, 15 mM MgCl2, 100 mM Tris-HCl pH 8.4
Storage -20 °C
Storage Comment at -20°C for two years
Expiry Date 24 months