TransScript® II One-Step gDNA Removal and cDNA Synthesis SuperMix

Details for Product No. ABIN5519514,
cDNA Synthesis (cDNA)
Purpose Unique genomic DNA remover is combined with TransScript® II First- Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis.
Brand TransScript®
Specificity Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
cDNA up to 15 kb.
Characteristics After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85 °C for 5 seconds.
Components TransScript® II RT/RI Enzyme Mix, gDNA Remover, 2xTS II Reaction Mix, Random Primer (0.1 μg/μL), Anchored Oligo(dT)20 Primer (0.5 μg/μL), RNase-free Water
Application Notes cDNA library construction, 3' and 5' RACE, Multiple copy and low copy gene detection, GC-rich or complex secondary structure RNA template

50 rxnsx20 μl Systems

Restrictions For Research Use only
Storage -20 °C
Storage Comment at -20°C for two years
Expiry Date 24 months