TransStart® FastPfu Fly DNA Polymerase (with 2.5 mM dNTPs)

Details for Product No. ABIN5519538,
Application
Polymerase Chain Reaction (PCR)
Options
Purpose TransStart® FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase.
Brand TransStart®
Specificity TransStart® FastPfu Fly DNA Polymerase has an extension rate of up to 6 kb/min. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.
Characteristics - TransStart® FastPfu Fly DNA Polymerase offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
- Extension rate is about 2-6 kb/min.
- PCR products can be directly cloned into pEASY®-Blunt vectors.
- Amplification of genomic DNA fragment up to 15 kb.
- Amplification of plasmid DNA fragment up to 20 kb.
Components DNA Polymerase, 5X Pfu Buffer, 2.5 mM dNTPs, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
Unit Definition One unit of TransStart® FastPfu Fly DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Application Notes High fidelity PCR, High yield and fast PCR, Blunt end cloning, Site-directed mutagenesis, Complex templates
Comment

TransStart® FastPfu Fly DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of TransStart® FastPfu Fly DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

Restrictions For Research Use only
Buffer Storage Buffer:50 mM Tris-HCl ( pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50 % glycerol
5xTransStart® FastPfu Fly Buffer: 100 mM Tris-SO4 pH 9.2, 50 mM (NH4)2SO4, 200 mM KCl, 10 mM MgSO4, 10 % Glycerol, others
Storage -20 °C
Storage Comment at -20°C for two years
Expiry Date 24 months