TransStart® KD Plus DNA Polymerase (with 2.5 mM dNTPs)

Details for Product No. ABIN5519548,
Polymerase Chain Reaction (PCR)
Purpose TransStart® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase.
Brand TransStart®
Specificity This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3'-5' exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
Characteristics - PCR products can be directly cloned into pEASY®-Blunt vectors.
- Amplification of genomic DNA fragment up to 15 kb.
- Amplification of plasmid DNA fragment up to 20 kb.
Components DNA Polymerase, 5X Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer, 50 mM MgSO4
Application Notes Fast, high specificity amplification, High fidelity, high yield amplification
Restrictions For Research Use only
Buffer Storage Buffer: 50 mM Tris-Cl ( pH 8.0),50 mM KCl,1 mM DTT,0.1 mM EDTA,Stabilizers,50 % glycerol.
5xTransStart® KD Plus Buffer with 20 mM MgSO4: 100 mM Tris-Cl ( pH 9.2),50 mM (NH4)2SO4,200 mM KCl,5 mM MgSO4,10 % glycerol, other.
Storage -20 °C
Storage Comment at -20°C for two years
Expiry Date 24 months