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TransStart® KD Plus DNA Polymerase (with 2.5 mM dNTPs)

PCR
Catalog No. ABIN5519548
  • Application
    Polymerase Chain Reaction (PCR)
    Purpose
    TransStart® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase.
    Brand
    TransStart®
    Specificity
    This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3'-5' exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
    Characteristics
    - PCR products can be directly cloned into pEASY®-Blunt vectors.
    - Amplification of genomic DNA fragment up to 15 kb.
    - Amplification of plasmid DNA fragment up to 20 kb.
    Components
    DNA Polymerase, 5X Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer, 50 mM MgSO4
  • Application Notes
    Fast, high specificity amplification, High fidelity, high yield amplification
    Restrictions
    For Research Use only
  • Buffer
    Storage Buffer: 50 mM Tris-Cl ( pH 8.0),50 mM KCl,1 mM DTT,0.1 mM EDTA,Stabilizers,50 % glycerol.
    5xTransStart® KD Plus Buffer with 20 mM MgSO4: 100 mM Tris-Cl ( pH 9.2),50 mM (NH4)2SO4,200 mM KCl,5 mM MgSO4,10 % glycerol, other.
    Storage
    -20 °C
    Storage Comment
    at -20°C for two years
    Expiry Date
    24 months
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