BST DNA Polymerase (Large Fragment)

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Application
Polymerase Chain Reaction (PCR)
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Purpose Bst DNA Polymerase, Large Fragment is a portion of DNA polymerase of Bacillus stearothermophilus, which catalyzes 5'→3' synthesis of DNA and strand displacement, but lacks 5'→3' and 3'→5' exonuclease activities.
Components Bst DNA Polymerase, Large Fragment 8.0 unit/μl, 10x BstPol Reaction Buffer, 100 mM MgSO4
Unit Definition One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C.
Application Notes Isothermal amplification (LAMP and RCA), DNA sequencing through high GC regions, Rapid Sequencing from nanogram amounts of DNA template
Comment

Bst Polymerase, Large Fragment is prepared from an E. coli strain containing the Bacillus stearothermophilus DNA Polymerase gene, lacking the 5'→3' exonuclease domain

Restrictions For Research Use only