FlashPfu DNA Polymerase

Details for Product No. ABIN5666439,
Polymerase Chain Reaction (PCR)
Purpose FlashPfu is a DNA polymerase for high-fidelity PCR. By fusing Pfu with a high affinity double-stranded DNA binding domain, we have dramatically increased its processivity and extension rate. FlashPfu DNA polymerase is 50-fold more accurate than Taq polymerase and 6-fold more accurate than Pfu polymerase.
Components FlashPfu DNA Polymerase 1 U/μl, 2x flashPfu PCR Buffer, 25 mM MgSO4
Unit Definition One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 75°C.
Application Notes High Fidelity PCR, Fast PCR- extension 15-30 s/kb, Long range PCR -genomic DNA ≤ 19 kb, lambda DNA ≤ 30 kb), Generation of PCR product for blunt-end cloning, Site-directed mutagenesis
Restrictions For Research Use only