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Eva Green dye is the next generation DNA-binding dye, it absorbs blue light (λmax = 497 nm) and emits green light (λmax=520 nm) when binding to dsDNA. The increase in fluorescent signal is directly proportional to the quantity of exponentially accumulating PCR product molecules (amplicons), thus direct detection of PCR product is monitored by measuring the increase in fluorescence caused by the binding of EVA Green dye to ds DNA.