Golden qPCR EVA Green Master Mix (2X) (Dye-Based)

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Application
Quantitative real-time PCR (qPCR)
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Purpose Golden qPCR EVA Green Master Mix(2x) is an optimal 2x concentrated solution of hot-start DNA polymerase, dNTPs, EVA Green dye, ROX and all other components required for quantitative real-time PCR, except DNA template and primers. This is a premixed, ready-to-use solution with high sensitivity and specificity.
Characteristics High sensitivity and specificity: The chemically modified hot-start DNA polymerase and the optimized buffer in the Mix eliminate non-specific amplification and formation of primer dimers.
Convenience: A premixed, ready-to-use solution with Eva Green I dye.
Compatible: The pre-added ROX dye allows for correction of well-to-well variation possibly caused by pipetting inaccuracies and fluorescence fluctuations. The mix is compatible with most instruments, including those not requiring a passive reference signal for data normalization.
Application Notes An initial denaturation step of 10 min at 95 °C to activate Hot Start Taq DNA polymerase is necessary.
ROX dye is pre-added in the master mix.
Comment

Eva Green dye is the next generation DNA-binding dye, it absorbs blue light (λmax = 497 nm) and emits green light (λmax=520 nm) when binding to dsDNA. The increase in fluorescent signal is directly proportional to the quantity of exponentially accumulating PCR product molecules (amplicons), thus direct detection of PCR product is monitored by measuring the increase in fluorescence caused by the binding of EVA Green dye to ds DNA.

Restrictions For Research Use only
Handling Advice Avoid repeated freeze-thaw cycle
Storage 4 °C,-20 °C
Storage Comment -20°C for 12 months 4°C for 2months