Golden qPCR SYBR Green Master Mix (2X)

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Application
Quantitative real-time PCR (qPCR)
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Purpose Golden qPCR SYBR Green Master Mix(2x) is an optimal 2x concentrated solution of hot-start DNA polymerase, MgCl2, dNTPs, SYBR Green I dye and all other components required for quantitative real-time PCR, except DNA template and primers. This is a premixed, ready-to-use solution with high sensitivity and specificity.
Characteristics High sensitivity and specificity. The chemically modified hot-start DNA polymerase and the optimized buffer in the Mix eliminate non-specific amplification and formation of primer dimers. This ensures reproducible, sensitive and specific quantification of template.
Convenience. A premixed, ready-to-use solution with SYBR Green I.
Slim well-to-well variation(without ROX)
Application Notes An initial denaturation step of 10 min at 95 °C to activate Hot Start Taq DNA polymerase is necessary.
ROX dye not provided.
Comment

SYBR Green I is a DNA-binding dye, it absorbs blue light (λmax = 497 nm) and emits green light (λmax = 520 nm) when binding to dsDNA.The increase in fluorescent signal is directly proportional to the quantity of exponentially accumulating PCR product molecules (amplicons), thus direct detection of PCR product is monitored by measuring the increase in fluorescence caused by the binding of SYBR Green I to dsDNA.

Restrictions For Research Use only
Handling Advice Avoid repeated freeze-thaw cycle
Storage 4 °C,-20 °C
Storage Comment -20°C for 12 months 4°C for 2months