Golden qPCR TaqMan Probe Master Mix (2X)

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Quantitative real-time PCR (qPCR)
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Purpose Golden qPCR TaqMan Probe Master Mix(2x)is a special mixture for Taqman probe real time PCR Which includes the hot-start DNA polymerase, PCR Buffer, MgCl2, dNTPs and all other components required for quantitative real-time PCR, except DNA template, primers and probes.
Characteristics High sensitivity and specificity. The chemically modified hot-start DNA polymerase and the optimized buffer in the Mix eliminate non-specific amplification and formation of primer dimers. This ensures reproducible, sensitive and specific quantification of template.
Convenience. A premixed, ready-to-use solution for Taqman probe real time PCR.
Slim well-to-well variation(without ROX)
Application Notes An initial denaturation step of 10 min at 95 °C to activate Hot Start Taq DNA polymerase is necessary.
ROX dye is not provided.

The PCR reaction exploits the 5' nuclease activity of DNA Polymerase to cleave a TaqMan probe which anneals within a DNA region amplified by a specific set of primers during PCR. The TaqMan probe contains a reporter dye at the 5' end of the probe and a quencher dye at the 3' end. The quencher dye quenches the fluorescence emitted by reporter dye via FRET when the probe is intact. During the PCR, as the DNA polymerase extends the primer and synthesizes the nascent strand, the 5' to 3' exonuclease activity of the DNA polymerase degrades the probe that has annealed to the template. Degradation of the probe releases the fluorophore from the reporter and breaks the quenching effect of the quencher. Hence, fluorescence detected in the quantitative PCR thermal cycler is directly proportional to the fluorophore released and the amount of DNA template present in the PCR.

Restrictions For Research Use only
Handling Advice Avoid repeated freeze-thaw cycle
Storage 4 °C,-20 °C
Storage Comment -20°C for 12 months 4°C for 2months