T4 DNA Ligase

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Ligation (Lig)
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Purpose T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Components T4 DNA Ligase 250 unit/μl, 10x T4 DNA Ligase Buffer
Unit Definition One unit of T4 DNA Ligase is defined as the amount of enzyme required to catalyze the ligation of greater than 90% of λ DNA-HindIII fragments in 20 μl at 16°C in 30 minutes. One unit of this enzyme corresponds to 0.008 Weiss units.
Application Notes Cohesive and blunt end DNA ligation, DNA fragment insertion into vectors, Linker or adaptor ligation to a DNA fragment

T4 DNA Ligase is prepared from an E. coli strain carrying the plasmid that enable highly expression of T4 DNA.

Restrictions For Research Use only