Taq DNA Polymerase

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Application
Polymerase Chain Reaction (PCR)
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Purpose Taq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.
Characteristics Taq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It has both 5'→3' polymerase and 5'→3' exonuclease activity, but lacks 3'→5' exonuclease activity. As most PCR products amplified with Taq DNA Polymerase have one A added at 3'-termini, the obtained PCR product can be directly used for cloning into T-Vector.
Components Taq DNA Polymerase 5 U/μl, 10xTaq PCR Buffer, 25 mM MgCl2
Unit Definition One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 75°C.
Application Notes General PCR, Colony PCR, Primer Extension
Comment

Taq DNA Polymerase is prepared from E. coli carrying a plasmid that encodes the Thermus aquaticus DNA polymerase gene.

Restrictions For Research Use only