Uracil-DNA Glycosylase (UDG)

Details for Product No. ABIN5666461,
Purpose The Uracil-DNA Glycocasylase (UDG) is expressed from recombinant clone of E. Coil, and the molecular weight is 25 kDa. The optimum reaction temperature of UDG is 50°C.
Characteristics The Uracil-DNA Glycocasylase (UDG) is designed to prevent contamination of PCR amplification products. One important function of this enzyme is to hydrolyzes uracil-glycosidic bonds at U-DNA sites in single- and double-stranded DNA, excising uracil and creating alkali- and heat-sensitive abasic sites in the DNA.
Unit Definition One unit of the enzyme is the amount that completely cleaves 1 μg ds-DNA with dU in one hour at 37°C in the condition of 20 mM Tris-Hcl (PH8.0), 1 mM EDTA, 10 mM NaCl.
Restrictions For Research Use only
Storage -20 °C,-80 °C
Storage Comment Store at -80°C for one year. Store at -20°C if using frequently.