Advance™ PCR Mix-Dye

Details for Product No. ABIN5684173,
Polymerase Chain Reaction (PCR)
Purpose 2X PCR MasterMix with dye
Characteristics AdvanceTM PCR Mix-Dye provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. AdvanceTM 2X PCR Mix is a ready-to-use mixture containing all the necessary reagents for highly successful amplification of DNA (highfidelity DNA Polymerase, deoxynucleotides, reaction buffer and a sophisticated blend of additives in a 2X concentration). The AdvanceTM 2X PCR Mix advanced buffer system not only tolerates A/T- and G/C-rich content, but also many PCR inhibitors commonly found in a typical DNA sample. AdvanceTM 2X PCR Mix is the most robust PCR Master Mix commercially available and will deliver an exceptional product yield with high fidelity. AdvanceTM DNA Taq Polymerase is a novel DNA polymerase with strategically engineered mutations resulting in a robust, high-fidelity polymerase. AdvanceTM DNA Taq polymerase has exceptional 3' to 5' exonuclease activity that endows it with superior accuracy over competitor polymerases. This novel enzyme has intrinsically high processivity and is engineered to have an improved binding affinity for DNA resulting in highly successful PCR. AdvanceTM 2X PCR Mix with dye contains an inert green dye mixture and a stabilizer which allow for direct loading of the final PCR products onto an agarose gel for analysis. The green dye resolves during gel electrophoresis into a turquoise band at ~4000 bp and a yellow band at the ~50 bp region. The resolving bands of dye allow easy visualization of the real time progress of the gel electrophoresis, and the yellow band indicates the migrating front of the gel. To set up a PCR reaction: Add DNA template, primers and water. Blunt-ended PCR products p to 13 kb in length can be amplified with AdvanceTM 2X PCR Mix.
Application Notes - Routine PCR amplification of DNA templates
- High-throughput PCR
- Robust amplification of AT- and GC-Rich sequences
- NGS Library construction

- Saves preparation time by combining AdvanceTM DNA Taq Polymerase, dNTPs and reaction buffer in a ready-to-use mixture
- Reduces the risk of contamination by decreasing the number of pipetting steps
- Provides consistent reaction performance and results
- Robust PCR performance with high yield and superb fidelity
- Compatible with plant DNA templates.
- High resistance to most PCR inhibitors commonly found in samples, including plant samples
- 2X Master Mix format for easy PCR setup
- Advanced formulation for Master Mix Stability

Protocol For difficult templates (A/T or G/C rich) and DNA samples containing PCR inhibitors
Restrictions For Research Use only
Storage 4 °C,-20 °C
Storage Comment Upon arrival, the AdvanceTM 2X PCR Mix should be stored at -20°C. AdvanceTM 2X PCR Mix is stable at 4°C for three months or for fifteen freeze-thaw cycles. For daily use, we recommend keeping an aliquot at 4°C. The AdvanceTM 2X Master Mix is stable for 1 year from the date of shipping when stored and handled properly. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months