BreezeTM PCR Mix with dye provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. BreezeTM 2X PCR Mix is a ready-to-use mixture containing BreezeTM DNA polymerase, dNTPs, and reaction buffer with proprietary additives in a 2X concentration. It contains all the necessary reagents for reproducible, efficient and high yield amplification of DNA. The Breeze™ Taq DNA polymerase is a uniquely engineered version of Taq DNA polymerase which allows it to achieve rapid PCR amplification. This member of BioVision's PCR polymerase product series is the perfect solution to ultimate speed and dramatically reduced reaction time. The 2X BreezeTM Master Mix with dye contains a green dye blend which resolves during gel electrophoresis into a turquoise band at ~4000 bp and a yellow band at the ~50 bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel. BreezeTM DNA polymerase is capable of an extension speed of 6 kb/min - 6 times faster than the regular Taq polymerase. This polymerase catalyzes the 5' - 3' synthesis of DNA. It possesses 5' - 3' exonuclease activity and lacks 3' - 5' proofreading exonuclease activity: making it the ideal choice for high through-put PCR. In addition, template-independent ""A"" can be attached at the 3' end of the PCR product which can then be cloned into TA cloning vectors for downstream applications.