Breeze™ PCR Mix

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Application
Polymerase Chain Reaction (PCR)
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Purpose 2X PCR MasterMix
Characteristics BreezeTM PCR Mix with dye provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. BreezeTM 2X PCR Mix is a ready-to-use mixture containing BreezeTM DNA polymerase, dNTPs, and reaction buffer with proprietary additives in a 2X concentration. It contains all the necessary reagents for reproducible, efficient and high yield amplification of DNA. The Breeze™ Taq DNA polymerase is a uniquely engineered version of Taq DNA polymerase which allows it to achieve rapid PCR amplification. This member of BioVision's PCR polymerase product series is the perfect solution to ultimate speed and dramatically reduced reaction time. The 2X BreezeTM Master Mix with dye contains a green dye blend which resolves during gel electrophoresis into a turquoise band at ~4000 bp and a yellow band at the ~50 bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel. BreezeTM DNA polymerase is capable of an extension speed of 6 kb/min - 6 times faster than the regular Taq polymerase. This polymerase catalyzes the 5' - 3' synthesis of DNA. It possesses 5' - 3' exonuclease activity and lacks 3' - 5' proofreading exonuclease activity: making it the ideal choice for high through-put PCR. In addition, template-independent ""A"" can be attached at the 3' end of the PCR product which can then be cloned into TA cloning vectors for downstream applications.
Application Notes Optimal working dilution should be determined by the investigator.
Comment

- Saves preparation time by combining BreezeTM Taq DNA Polymerase, dNTPs and reaction buffer in a ready-to-use mixture
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by 80%
- Reduces the risk of contamination by decreasing the number of pipetting steps
- Provides consistent reaction performance and results

Protocol For rapid PCR amplification thereby dramatically reducing the reaction time
Restrictions For Research Use only
Storage 4 °C,-20 °C
Storage Comment Upon arrival, the BreezeTM 2X PCR Mix should be stored at -20°C. BreezeTM PCR Mix with dye are stable at 4°C for three months or for fifteen freeze-thaw cycles. For daily use, we recommend keeping an aliquot at 4°C. The BreezeTM 2X Master Mix is stable for 1 year from the date of shipping when stored and handled properly. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months