Distant™ PCR Mix-Dye

Details for Product No. ABIN5684177, Supplier: Log in to see
Application
Polymerase Chain Reaction (PCR)
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Purpose 2X PCR MasterMix with dye
Characteristics DistantTM PCR PCR Mix-Dye, provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. DistantTM 2X PCR Mix is a ready-to-use mixture containing DistantTM DNA Taq Polymerase, dNTPs, and reaction buffer with proprietary additives in a 2X concentration. It contains all the necessary reagents for an efficient, high-yield amplification of DNA. The 2X PCR DistantTM Master Mix with dye contains a green dye blend which resolves during gel electrophoresis into a turquoise band at ~4000 bp and a yellow band at the ~50 bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, and the yellow band indicates the migrating front in the gel. DistantTM DNA Taq Polymerase, with its superior fidelity and extreme robustness, is one of the best-performing DNA polymerases available on the market. With its monomeric, unique topological structure, DistantTM DNA Taq Polymerase possesses enhanced processivity, elevated yield, boosted speed, extended amplification length, and improved difficult template tolerance.
This high performing DistantTM Taq Polymerase and its Master Mix are the ideal choice for any PCR application, especially for cloning difficult templates, amplifying A/T- and G/C-rich sequences, and replicating long amplicons. With over 50X more accuracy than Taq DNA polymerase, DistantTM Taq DNA Polymerase and its Master Mix can consolidate all PCR protocols and reactions into one simple, efficient, and extremely reliable PCR system. DistantTM DNA Polymerase generates blunt-end PCR products and has both 5'-3' polymerase activity and 3'-5' proofreading exonuclease activity.
Application Notes - High-throughput PCR
- Robust amplification of AT- and GC-Rich sequences
- RACE
- NGS Library construction
Comment

- Saves preparation time by combining DistantTM DNA Taq Polymerase, dNTPs and reaction buffer in a ready-to-use mixture
- Reduces the risk of contamination by decreasing the number of pipetting steps
- Provides consistent reaction performance and results
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by up to 70%
- Robust and high yield across a wide range of templates
- Efficient amplification of DNA templates up to 15kb

Protocol For cloning of difficult templates (A/T or G/C rich) and long amplicons
Restrictions For Research Use only
Storage 4 °C,-20 °C
Storage Comment Upon arrival, the DistantTM PCR Mix should be stored at -20°C. 2X PCR DistantTM Master Mix with dye is stable at 4°C for three months or for fifteen freeze-thaw cycles. For daily use, we recommend keeping an aliquot at 4°C. The DistantTM 2X PCR Mix with dye is stable for 1 year from the date of shipping when stored and handled properly. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months