Evo™ RT Mastermix

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Purpose 5X All-In-One RT MasterMix
Characteristics EvoTM Reverse Transcriptase is an optimized and evolved (EvoTM) mutational derivative of the original Reverse Transcriptase (RTase) enzyme representing the best-performing RTase on the market. This enzyme catalyzes the synthesis of complementary DNA strands from single-stranded RNA or DNA templates. Due to a series of mutations introduced within the RNase H domain of this enzyme, there is no detectable RNase H activity associated with the enzyme. The lack of RNase H activity helps to eliminate RNA degradation during first-strand cDNA synthesis, resulting in better yield and length of cDNA synthesized (up to 9 kb). Furthermore, Evo™ RTase contains an additional fidelity enhancing subunit which drastically enhances accuracy in reverse transcription. EvoTM Reverse Transcriptase is the ultimate solution to synthesize cDNA from only 0.1 pg of RNA
Components EvoTM Reverse Transcriptase (200 U/μl), 5X RT buffer
Application Notes - Synthesizing cDNA from ssRNA
- DNA primer extension
- Sequencing dsDNA
- Constructing cDNA library
- Producing template for use in RT PCR
- Labelling 3'-end of duplex DNA via end filling reactions
- Generating probes for hybridization
Protocol An optimized mutational derivative of the original Reverse Transcriptase and A novel recombinant reverse transcriptase representing the best-performing RTase on the market
Restrictions For Research Use only
Storage -20 °C
Storage Comment Store all components at -20°C in a non-frost-free freezer. All components are stable for 1 year from the date of shipping when stored and handled properly. Avoid repeated freeze-thaw cycles to retain maximum performance. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months