Evo™ RT Mastermix (with gDNA Removal)

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Purpose 5X All-In-One RT MasterMix
Characteristics EvoTM RT Mastermix (with genomic DNA Removal Kit) is a 5X all-in-one RT Mastermix that provides a convenient and highly efficient method for first-strand cDNA synthesis with an additional genomic DNA removal step included. The presence of contaminating genomic DNA (gDNA) in RNA preparations is often a significant problem for downstream applications, leading to false-positive signals and misinterpretation of gene expression levels. Effective elimination of gDNA is therefore the most reliable method to ensure accurate experimental results. The Genomic DNA Removal Kit provided will effectively remove contaminating gDNA from the sample in under 10 minutes, without any heating or organic extraction steps which can result in damage to the RNA template. The treated gDNA-free RNA can then be directly reverse-transcribed into cDNA using the 5X All-In-One RT Mastermix. The 5X RT Mastermix is a ready-to-use formulation of all the reagents necessary for first-strand cDNA synthesis. Coupled together, this complete system provides the ultimate convenience in generating high-quality cDNA suitable for a wide range of downstream applications. Note: Upon completion of the first-strand cDNA synthesis, the cDNA product can be directly applied as a template in a standard PCR/QPCR.
Components Reaction Mix (4X), Reaction Stopper (5X), 5X All-In-One RT Mastermix, Nuclease-free H2O
Application Notes - Generation of templates for use in RT PCR and QRT PCR
- cDNA synthesis from ssRNA
- cDNA library construction
- Generation of probes for hybridization
- DNA primer extension
Comment

- Efficient ds and ssDNA digestion in under 10 min
- Maximal RNA protection- No heating or organic extraction steps required
- Fewer handling errors, with only 1 liquid transfer step for reverse transcription
- Streamlined protocol suitable for high-throughput application
- Simple set-up for any RNA template
- High reproducibility and excellent cDNA yield

Protocol An optimized mutational derivative of the original Reverse Transcriptase and A novel recombinant reverse transcriptase representing the best-performing RTase on the market
Restrictions For Research Use only
Storage -20 °C
Storage Comment Store all components at -20°C in a non-frost-free freezer. All components are stable for 1 year from the date of shipping when stored and handled properly. Avoid repeated freeze-thaw cycles to retain maximum performance. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months