Fire Start™ PCR Mix

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Application
Polymerase Chain Reaction (PCR)
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Purpose 2X PCR MasterMix
Characteristics Fire StartTM PCR Mix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Fire StartTM 2X PCR Mix is a ready-to-use mixture of high quality Fires StartTM Taq DNA Polymerase, deoxynucleotides, and reaction buffer in a 2X concentration. It contains all the necessary reagents for the amplification of DNA. Fire StartTM Taq DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures and thus eliminates the problems with non-specific amplification and primer-dimer formation. During the initial denaturation step at 94 °C, the antibody dissociates from Fire StartTM Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability. Thus, Fire StartTM Taq DNA Polymerase offers improved yield of desired PCR products. To set up a PCR reaction: add DNA template, primers and water. Additionally, the Fire StartTM Master Mix with dye contains an inert green dye blend and a stabilizer which allows for direct loading of the final products onto a gel for analysis. The green dye blend resolves during gel electrophoresis into a turquoise band at ~4000 bp and a yellow band at the ~50 bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel.
Application Notes - Assays with prolonged reaction setup or liquid handling
- Multiplex PCR
- Specific amplification of difficult templates (i.e. G-C rich)
- Low copy PCR assays
- TA Cloning
Comment

- Saves preparation time by combining Fire StartTM Taq DNA Polymerase, dNTPs and reaction buffer in a ready-to-use mixture
- Reduces the risk of contamination by decreasing the number of pipetting steps
- Provides consistent reaction performance and results
- The highest specificity with minimal background
- Superior performance
- Improved yield of desired product

Protocol Contains high-quality Taq DNA Polymerase that blocks the polymerase activity at low temperatures thereby reducing non-specific product formation
Restrictions For Research Use only
Storage 4 °C,-20 °C
Storage Comment Upon arrival, the Fire StartTM PCR Mix-Dye should be stored at -20°C. Fire StartTM PCR Mix with dye are stable at 4°C for three months or for fifteen freeze-thaw cycles. For daily use, we recommend keeping an aliquot at 4°C. The Fire StartTM Master Mix-Dye is stable for 1 year from the date of shipping when stored and handled properly. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months