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Characteristics
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Fire StartTM PCR Mix-Dye provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Fire StartTM 2X PCR Mix is a ready-to-use mixture of high quality Fires StartTM Taq DNA Polymerase, deoxynucleotides, and reaction buffer in a 2X concentration. It contains all the necessary reagents for the amplification of DNA. Fire StartTM Taq DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures and thus eliminates the problems with non-specific amplification and primer-dimer formation. During the initial denaturation step at 94 °C, the antibody dissociates from Fire StartTM Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability. Thus, Fire StartTM Taq DNA Polymerase offers improved yield of desired PCR products. To set up a PCR reaction: add DNA template, primers and water. Additionally, the Fire StartTM Master Mix with dye contains an inert green dye blend and a stabilizer which allows for direct loading of the final products onto a gel for analysis. The green dye blend resolves during gel electrophoresis into a turquoise band at ~4000 bp and a yellow band at the ~50 bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel.
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