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Gene Snipper™ Cas9 NLS Enzyme

Catalog No. ABIN5684191
Plus shipping costs $45.00 and $24.00 dry ice
0.25 nmol ABIN5684191
0.25 nmol ABIN5684191
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
  • Application
    Genome Editing with Engineered Nucleases (GEEN)
    Cas9 NLS Nuclease Protein
    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. Cas9 Nuclease nuclear localization sequence (NLS), S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. Guided by a targetspecific, single guide RNA (sg RNA), the Cas9 Nuclease NLS Protein serves to cleave both strands of a DNA duplex upon recognition of the target sequence by the sg RNA. The resulting double-stranded break gets repaired by the nonhomologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Cas9 Nuclease NLS contains a SV40 T antigen NLS on the C-terminus of the protein. Incorporation of a nuclear localization signal (NLS) aids delivery to the nucleus, thus increasing the rate of genomic DNA cleavage.
    Cas9 NLS, 10X Cas9 Reaction Buffer
  • Application Notes
    Optimal working dilution should be determined by the investigator.

    Recombinant Streptococcus pyogenes Cas9 nuclease NLS protein (CRISPR associated protein 9) purified from E. coli

    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Store all components at -20°C. Avoid repeated freeze/thaw cycles. All components are stable for 1 year from the date of shipping when stored and handled properly
    Expiry Date
    12 months
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