Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@genomics-online.com

Gene Snipper™ Cas9 NLS Enzyme

GEEN
Catalog No. ABIN5684191
$410.00
Plus shipping costs $45.00 and $24.00 dry ice
0.25 nmol ABIN5684191
0.25 nmol ABIN5684191
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
  • Application
    Genome Editing with Engineered Nucleases (GEEN)
    Purpose
    Cas9 NLS Nuclease Protein
    Characteristics
    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. Cas9 Nuclease nuclear localization sequence (NLS), S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. Guided by a targetspecific, single guide RNA (sg RNA), the Cas9 Nuclease NLS Protein serves to cleave both strands of a DNA duplex upon recognition of the target sequence by the sg RNA. The resulting double-stranded break gets repaired by the nonhomologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Cas9 Nuclease NLS contains a SV40 T antigen NLS on the C-terminus of the protein. Incorporation of a nuclear localization signal (NLS) aids delivery to the nucleus, thus increasing the rate of genomic DNA cleavage.
    Components
    Cas9 NLS, 10X Cas9 Reaction Buffer
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Recombinant Streptococcus pyogenes Cas9 nuclease NLS protein (CRISPR associated protein 9) purified from E. coli

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Store all components at -20°C. Avoid repeated freeze/thaw cycles. All components are stable for 1 year from the date of shipping when stored and handled properly
    Expiry Date
    12 months
You are here:
help Support