Gene Snipper™ Cas9 NLS

Details for Product No. ABIN5684192, Supplier: Log in to see
Application
Genome Editing with Engineered Nucleases (GEEN)
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Purpose Cas9 NLS Nuclease Protein
Characteristics The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. Cas9 Nuclease nuclear localization sequence (NLS), S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. Guided by a targetspecific, single guide RNA (sg RNA), the Cas9 Nuclease NLS Protein serves to cleave both strands of a DNA duplex upon recognition of the target sequence by the sg RNA. The resulting double-stranded break gets repaired by the nonhomologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Cas9 Nuclease NLS contains a SV40 T antigen NLS on the C-terminus of the protein. Incorporation of a nuclear localization signal (NLS) aids delivery to the nucleus, thus increasing the rate of genomic DNA cleavage.
Components Cas9 NLS, 10X Cas9 Reaction Buffer
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Recombinant Streptococcus pyogenes Cas9 nuclease NLS protein (CRISPR associated protein 9) purified from E. coli

Restrictions For Research Use only
Storage -20 °C
Storage Comment Store all components at -20°C. Avoid repeated freeze/thaw cycles. All components are stable for 1 year from the date of shipping when stored and handled properly
Expiry Date 12 months