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Gene Snipper™ Cas9 Protein Enzyme

GEEN
Catalog No. ABIN5684201
$405.00
Plus shipping costs $45.00 and $24.00 dry ice
0.25 nmol ABIN5684201
0.25 nmol ABIN5684201
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
  • Application
    Genome Editing with Engineered Nucleases (GEEN)
    Purpose
    Cas9 Nuclease Protein
    Characteristics
    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR adaptive immunity system in Streptococcus pyogenes, among other bacteria. S. pyogenes utilizes Cas9 to memorize and later interrogate and cleave foreign DNA such as invading bacteriophage DNA or plasmid DNA. Cas9 Nuclease protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (sg RNA) of the CRISPR/Cas9 system and unwinds the genomic DNA duplex and cleaves both strands upon recognition of the target sequence by the sg RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene.
    Components
    Cas9, 10X Cas9 Reaction Buffer
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Recombinant Streptococcus pyogenes Cas9 (CRISPR associated protein 9) nuclease protein purified from E. coli

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Store all components at -20°C. Avoid repeated freeze/thaw cycles. All components are stable for 1 year from the date of shipping when stored and handled properly
    Expiry Date
    12 months
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