Gene Snipper™ Cas9 Protein

Details for Product No. ABIN5684201,
Application
Genome Editing with Engineered Nucleases (GEEN)
Options
Purpose Cas9 Nuclease Protein
Characteristics The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR adaptive immunity system in Streptococcus pyogenes, among other bacteria. S. pyogenes utilizes Cas9 to memorize and later interrogate and cleave foreign DNA such as invading bacteriophage DNA or plasmid DNA. Cas9 Nuclease protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (sg RNA) of the CRISPR/Cas9 system and unwinds the genomic DNA duplex and cleaves both strands upon recognition of the target sequence by the sg RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene.
Components Cas9, 10X Cas9 Reaction Buffer
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Recombinant Streptococcus pyogenes Cas9 (CRISPR associated protein 9) nuclease protein purified from E. coli
Restrictions For Research Use only
Storage -20 °C
Storage Comment Store all components at -20°C. Avoid repeated freeze/thaw cycles. All components are stable for 1 year from the date of shipping when stored and handled properly
Expiry Date 12 months