Image Distant™ PCR Mix

Details for Product No. ABIN5684203, Supplier: Log in to see
Application
Polymerase Chain Reaction (PCR)
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Purpose 2X PCR MasterMix
Characteristics Image DistantTM PCR Mix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Image DistantTM 2X PCR Mix is a ready-to-use mixture containing BioVision's DistantTM DNA Taq Polymerase, deoxynucleotides, and reaction buffer in a 2X concentration. It contains all the necessary reagents for the amplification of DNA along with an inert blue dye, a proprietary amount of Image Safe reagent and a stabilizer which allow direct loading of the final products onto an ethidium bromide-free gel for analysis. To set up a PCR reaction: add DNA template, primers and water. PCR products that are amplified up to 10 kb in length with Image DistantTM 2X PCR Mix are blunt ended. The Image Safe Reagent is proven to be non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.
Application Notes - High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by up to 70 %
- Robust and high yield across a wide range of templates
- Efficient amplification of DNA templates up to 15kb
Comment

- Saves preparation time by combining DistantTM DNA Taq Polymerase, dNTPs and reaction buffer in a ready-to-use mixture
- Reduces the risk of contamination by decreasing the number of pipetting steps
- Provides consistent reaction performance and results
- Replaces the toxic ethidium bromide in gel analysis.
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by up to 70%
- Robust and high yield across a wide range of templates
- Efficient amplification of DNA templates up to 15kb

Restrictions For Research Use only
Storage 4 °C,-20 °C
Storage Comment Upon arrival, the Image DistantTM 2X Master Mix should be stored at -20°C. Image DistantTM 2X PCR Master Mix is stable at 4°C for three months or for fifteen freeze-thaw cycles. For daily use, we recommend keeping an aliquot at 4°C. The Image DistantTM 2X PCR Mix is stable for 1 year from the date of shipping when stored and handled properly. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months