Jade Express™ Mix

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Application
Quantitative real-time PCR (qPCR)
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Purpose 2X qPCR Master Mix
Characteristics Jade ExpressTM Mix provides a convenient, reliable and robust set-up for performing for extremely rapid quantitative PCR (real-time analysis) of DNA samples in a premixed and optimized format. Designed specifically for the aforementioned niche of application, the components of Jade ExpressTM 2X Master Mix promise top notch performance with respect to sensitivity, signal-to-noise ratio and elimination of primer dimers. The use of TaqFast DNA Polymerase in Jade ExpressTM 2X QPCR Master Mix shortens the reaction time to about 1/5 of standard QPCR time. Available with the option of ROX or fluorescein as the internal passive reference dye, Jade ExpressTM 2X QPCR Master Mix offers unparalleled performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. In light of the fact that the QPCR instruments can vary from user to user, offers the Jade ExpressTM 2X Master Mix in a range of formulations, each of which has been carefully optimized to confer the best performance according to the make and model of a QPCR machine. Please use QPCR Mix that will be most compatible with your choice of a particular instrument/model. Jade ExpressTM 2X Master Mix (Cat# M1109-500) is compatible with BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™, Roche LightCycler® (2.0, 1.5, 480, 1536, Nano), MJ Research Opticon™, Opticon™ 2, Chromo® 4, Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600) QPCR instruments.
Components Jade ExpressTM 2X QPCR Master Mix is a 2X concentrated mix of TaqFast DNA Polymerase, dNTPs, MgCl2, fluorescent dye (detection), reference dye and proprietary buffer components.
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Jade Express™ Mix

Protocol For ultrafast PCR, conferring a significant reduction to the overall QPCR quantification and detection time thus streamlining the experiment through cost and labor saving.
Restrictions For Research Use only
Storage 4 °C,-20 °C
Storage Comment Upon arrival, the Jade ExpressTM 2X Master Mix should be stored at -20°C and protected from light. After each experiment, the leftover mix (completely thawed and thoroughly homogenized) can be stored at 4°C if it is to be used within the next 3 months. Avoid repeated freeze-thaw cycles to retain maximum performance. The Jade ExpressTM 2X Master Mix is stable for 1 year from the date of shipping when stored and handled properly. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months